摘要
目的利用所构建的具复制能力的HBV重组腺病毒感染体外培养肝细胞,观察HBV的表达情况,从而初步了解该重组HBV腺病毒的生物学特性。方法以25pfu/细胞的重组腺病毒感染3种肝源性细胞株:人肝癌细胞株HepG2、人正常肝细胞株L02和禽肝细胞株LMH。感染3d后以RT-PCR检测3种感染肝细胞中的HBV mRNA;HBV荧光定量PCR检测培养上清中HBVDNA;ELISA检测培养上清中HBsAg和HBeAg。结果3种被感染肝细胞均被检测到HBV特异的mRNA;培养上清中HBV DNA、HBsAg和HBeAg的表达量随时间而增加。结论重组腺病毒可以介导HBV在该3种不同来源的肝细胞中建立HBV的感染状态。
Objective To study hepatitis B virus （HBV） expression in 3 hepatocytes infected with recombinant adenovirus containing 1.2-copy HBV DNA. Methods A chicken hepatoma cell line and two human hepatocytes were infected by the recombinant adenovirus containing 1.2-copy HBV DNA at 25 pfu/cell. HBV-specific mRNA was detected by RT-PCR 3 days after the infection, and HBsAg and HBeAg were detected by ELISA and HBV DNA by real-time PCR daily after the infection. Results HBV mRNA expression was detected in all the 3 cells after recombinant adenovirus infection, and the quantities of HBV DNA and HBV antigens in the culture supernatant increased with the passage of time. Conclusion Infection with the recombinant adenovirus containing 1.2-copy HBV DNA can mediate HBV infection in the 3 cells in vitro.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第4期738-740,744,共4页
Journal of Southern Medical University
基金
广东省自然科学基金(04300689)
广东省科技计划项目(2005B10401034)
关键词
乙型肝炎病毒
重组腺病毒
肝细胞
hepatitis B virus
recombinant adenovirus
hepatocyte
