摘要
目的研究乙型肝炎病毒(HBV)基本核心启动子(BCP)区及前C(PreC)区基因的变异情况,并且分析其发生频率、分布规律及其与临床病情的关系。方法应用套式PCR扩增nt1660-1935的HBV DNA片段及PCR产物直接测序的方法检测BCP区及Pre-C区基因的变异情况。结果130例乙型肝炎患者标本中,BCP区nt1762/nt1764发生双突变者75例(57.69%),Pre-C区nt1896发生突变者20例(15.38%),并且这两种突变在重型肝炎组(75.00%)及肝硬化组(72.22%)所占比例均明显高于慢性肝炎组(52.56%);此外,在BCP区及Pre-C/C区还发现了一些突变频率较高的其他突变位点(如nt1753,nt1766,nt1846,nt1899,nt1915),其中nt1753突变仅发生于有nt1762/1764双突变的患者,与重型肝炎及肝硬化的发生有一定关系;在BCP区有4处核苷酸突变共存者10例,其中重型肝炎组(12.50%)及肝硬化组(13.88%)所占比例明显高于慢性肝炎组(5.12%)。结论HBV BCP区nt1762/nt1764双突变和Pre-C区nt1896突变与肝炎的活动、重型化及肝硬化的发生有关;BCP区其他位点的突变共存对肝炎病情的进展也有重要影响。
Objective To investigate the gene mutations in the regions of precore（ Pre C） and basic core promoter（BCP） of HBV DNA and its clinical significance. Methods The nt1660 - 1935 segment of HBV-DNA was amplified with nested PCR in 130 cases of he- patitis B. And the PCR product was sequenced. Results The nt1762/1764 double mutations were found in 75 cases and nt1896 mu- tation in 20 cases. The frequencies of nucleotides 1762/1764 mutations were higher in the cases of chronic hepatitis, severe hepatitis and liver cirrhosis than that of acute hepatitis. The frequency of pre-C nt1896 mutation was less than 1762/1764 double mutation in 130 cases, and this mutation was mainly found in the cases of severe liver disease and liver cirrhosis. In addition, several other sites （nt1753, nt1766, nt1846, nt1899, nt1915 ）with higher frequency of mutation in BCP and Pre-C regions were found, among which nt1753 mutation was probably associated with the severity of liver disease. The frequency of 4-site mutation in BCP was higher in severe liver diseases and liver cirrhosis than in chronic hepatitis~ Conclusion The mutations of nt1762/1764 in BCP and nt1896 in Pre-C region are both related to the aggravation of chronic liver disease. Besides, several other mutation sites found in BCP region may also be associated with the progression of liver disease.
出处
《山西医科大学学报》
CAS
2009年第4期302-307,共6页
Journal of Shanxi Medical University
基金
山西省留学回国人员基金资助项目(200156)
