摘要
Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover pretreatment liquid, whose fermentable sugar was from hemicellulose hydrolysis only, consisting of 81.16% xylose and 15.27% glucose, was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen （HLC） expression enhanced by 50.00% and 63.71% xylose consumption.
Pretreatment of the corn stover powder by dilute sulphuric acid(solid-liquid ratio 1:20) at 130°C for 30 min was carried out with 89.09% of the hemicellulose removed.After filtration,the xylose-rich corn stover pretreatment liquid,whose fermentable sugar was from hemicellulose hydrolysis only,consisting of 81.16% xylose and 15.27% glucose,was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen(HLC) expression enhanced by 50.00% and 63.71% xylose consumption.
基金
Supported by the Agriculture Application Investigation and Improvement Item of New Countryside Construction and Promotion Project of the Bureau of Science and Technology in Xi’an (NC08005)
