摘要
目的：建立了高效液相色谱法（ＨＰＬＣ）配合电化学检测器（ＥＣＤ）同时分析组织和体液中的组织胺（Ｈｉｓ）和去甲肾上腺素（ＮＡ）的方法。方法：用邻苯二甲醛（ＯＰＡ）和２巯基乙醇（２－ＭＥ）进行柱前衍生，衍生产物在离子对试剂辛基磺酸钠的作用下，采用ＰａｔｉｓｉｌＯＤＳ柱，乙酸等试剂组成缓冲溶液与乙腈按７∶３配成流动相，３甲基组织胺（３ＭＨｉｓ）为内标，检测器电位０．７Ｖ，结果：用该方法对小鼠心脏组织和血液中的Ｈｉｓ和ＮＡ进行同时测定，得到了满意的分离和检测结果，最低检测量约为１５ｐｇ（Ｓ／Ｎ＝３）。结论：这个研究提示，用一些特殊试剂与样品产生衍生物，是得到满意的分离结果而不影响灵敏度的途径。
Objective: This paper reports that a reversed phase high performance liquid chromatographic(HPLC) method had been established for the simultaneous separation and quantitative determination of histamine(His)and noradrenaline(NA).Methods: The method is simple and reliable.The isoindole products formed by precolume derivatization of His and NA with ophthalaldehyde(OPA) and 2mercaptoethanol(2ME),the analysis was carried out with Patisil ODS column,acetate buffer(pH=4.90)acetonitrile(7∶3) as mobile phase and 3methylhistamine(3M His) as internal standard.The detector potential was+0.7V for both compounds to keep background noise minimal.Results: Yielded a linear relationship of detection between the electrochemical signal and the compound content,A minimum detectable limit of 15 pg(S/N=3).An example of a practical application of the method to the determination of His and NA in rat cardiac tissus or humour is presented.Conclusion: The study suggests that derivatization of compounds with some special agents resultion in electrochemically active derivatives,may also be an approach sometimes required toachieve satisfactory separation without sacrificing detection sensitivity
出处
《河南医学研究》
CAS
1998年第1期6-9,共4页
Henan Medical Research
基金
河南省医学科学院科研基金
关键词
高效液相色谱
组织胺
去甲肾上腺素
柱前衍生
HPLC
electrochemical detection
histamine
noradrenaline
precolume derivatization
