摘要
[目的]研究含氟茶浸泡液的细胞毒性和致突变性,探讨高氟茶对中国仓鼠肺成纤维细胞(V79细胞)的遗传毒作用。[方法]选取绿茶A、绿茶B、对照茶为研究对象,分别制备其茶浸泡液。将上述3种茶浸泡液分别设立0.63、1.25、2.50、5.00、10.00、20.00、40.00g/L7个质量浓度组,对V79细胞分别进行2、4、8、12、24h染毒。采用甲基四唑蓝(MTT)法检测3种茶浸泡液对V79细胞的毒性;运用鼠伤寒沙门菌回复突变试验(Amestest)检测3种茶浸泡液的致突变性。[结果]绿茶A、绿茶B和对照茶浸泡液中氟化物的浓度分别为2330、1390、7.53mg/kg,绿茶A、绿茶B的氟含量均超过了中国农业行业标准(NY659—2003)规定茶叶中氟化物(以F-计)≤200mg/kg的标准。在MTT实验中,3种茶浸泡液对细胞活性的抑制作用具有明显的时间-效应(P<0.05)和剂量-效应关系(P<0.05)。绿茶A、绿茶B的细胞存活率明显低于对照茶(P<0.05)。在作用4￣24h时,绿茶A的细胞存活率低于绿茶B(P<0.05)。分析3种茶不同培养时间的半数抑制浓度(IC50)发现,3种茶组间IC50的差异均有统计学意义(P<0.05),绿茶A、绿茶B、对照茶不同培养时间平均IC50分别为20.26、23.24、28.18g/L。在Ames试验中,3种茶浸泡液在8￣5000μg/皿的浓度上,4菌株回变菌落数与相应的阴性对照组之间的差异均有统计学意义。[结论]绿茶A、B浸泡液对V79细胞活性均有抑制作用,其细胞毒性均明显高于对照茶。未见高氟茶浸泡液有致突变作用。绿茶A、B对V79细胞的毒性比对照组大可能与它含氟量高有关。
[Objective] To explore the cytotoxicity and mutagenicity of tea containing rich fluoride, and to study the genotoxicity of the same tea on Chinese hamster lung fibroblast cell （V79cell）. [ Methods ] V79 cells were treated with the suspension of green tea A, green tea B, and the control tea at the concentrations of 0.63, 1.25, 2.5, 5, 10, 20, and 40g/L for 2, 4, 8, 12, and 24 h. Methyl tetrazolium test（MTT）was used to detect the cytotoxicity of tea on V79 cell line. Salmonella typhimurium microsoml enzyme test （ Ames test ） was used to detect the mutagenicity of the three kinds of tea. [ Results ] The fluoride concentrations of green tea A, B and the control tea were 2330, 1 390, and 7.53 mg/kg, in which the former two exceeded the national standard （ NY659-2003 ）. In MTT test, viability inhibition could be induced by all the three kinds of tea. There were significant logarithmic time-effect and dose-effect dependent relationships. The cell survival rates of the green tea A, B were significantly less than the control tea （ P 〈 0.05 ）. The cell survival rate of the green tea B was significantly higher than that of the green tea A（ P 〈 0.05 ）at 4 hours-24 hours exposure. Based on the median lethal concentrations（ IC50）of the tea, there were significant differences among the three tea groups（ P 〈 0.05 ）. The average IC50 of green tea A, green tea B and control tea were 20.36, 23.24, and 28.18g/L respectively. In Ames test, at the concentrations of 8-5000 μg/dish, there were no significant differences in the bacteria colony amount between four strain and the corresponding negative control group. [Conclusion] Two kinds of test tea could induce viability inhibition. The cytotoxicity of the green tea A, B were significantly higher than the control tea. No mntagenicity on the tea containing rich fluoride was found in Ames test. The eytotoxicity of green tea A, B on V79 cell was associated with the high fluoride level in the test green tea.
出处
《环境与职业医学》
CAS
北大核心
2009年第2期166-169,174,共5页
Journal of Environmental and Occupational Medicine
基金
常熟市社会发展基金(编号:C2006060)
