摘要
从１２例骨肉瘤患者手术切除的实体瘤中分离肿瘤浸润淋巴细胞（ＴＩＬ），从患者引流淋巴结中分离淋巴细胞（ＬＮＬ），以ｒＩｌ—２激活培养，并以４小时５１Ｃｒ释放试验测定ＴＩＬ和ＬＮＬ体外抗瘤活性。结果表明：１２例骨肉瘤患者的ＴＩＬ培养１５～２５天，对Ｋ５６２和ＬｉＢｒ靶细胞（效靶比２５∶１）平均杀伤活性分别为５９．７±１２．６％和４４．２±１０．２％，激活的ＬＮＬ对Ｋ５６２和ＬｉＢｒ细胞的杀伤活性分别为５５．２±１１．６％和４０．８±９．１％，二者对Ｋ５６２和ＬｉＢｒ细胞杀伤活性相差不显著（Ｐ＞０．０５）。其中６例患者ＴＩＬ和ＬＮＬ对自身肿瘤细胞杀伤活性分别为３８．８±９．５％和３５．５±７．６％，二者相差不显著（Ｐ＞０．０５）。
Tumorinfiltrating Iymphcytes (TIL) and Iymph node Iymphocytes (LNL) were respectively separated from tumor cells in resected solid tomors and drain of lymph node in 12 patients with osteosarcoma.The TIL and LNL were cultivated in the presence of rIL2 for 15～20 days,and their antineoplastic activity in vitro was detected by four hour 51 Cr releasing assay.The results showed that the average killing activies of rIL2activated TIL and LNL to the target cells K562 and LiBr lines were 59.7±12.6%,44.2±10.2%and 55.2±11.6%,40.8±9.1%,respectively,the ratio of effctor cells to target cells being 25∶1.There was no significant difference in killing activity between the TIL and LNL (P>0.05).In 6 of the 12 patients,the killing activities of TIL and LNLto autologous tumor cells were 38.8±9.5% and 35.5±7.6% respectively,and also no significant difference between TIL and LNL(P>0.05).It was indicated that LNL activated by rIL2 have the same killing activity as TIL.It would provide a reference for clinical treatment of tumor patients with LNL.
出处
《承德医学院学报》
1998年第1期12-14,共3页
Journal of Chengde Medical University
