摘要
目的观察辐射对宫颈癌细胞表皮生长因子受体（EGFR）核转运的影响，并初步探讨EGFR核转运在宫颈癌细胞辐射抵抗当中的作用。方法通过免疫印记检测法分析了EGFR在宫颈腺癌细胞HeLa和Siha以及宫颈鳞癌细胞Caski中的表达，以及X线照射4Gy后Caski细胞核内EGFR和细胞浆内EGFR不同时间点的表达。并且分析了Cetuximab（C225）对辐射诱导的Caski细胞EGFR核表达影响及其生存分数的影响。结果3株宫颈癌细胞中Caski细胞系EGFR呈高表达。受照的Caski细胞核内EGFR受体表达随时间增多，胞质内EGFR受体减少。C225预处理后，辐射诱导的EGFR核表达明显降低并且生存分数降低。结论辐射能够诱导宫颈癌细胞EGFR核转运，并且EGFR核表达可能和放疗抵抗相关，核内EGFR在宫颈癌细胞放疗抵抗中的作用尚需进一步研究。
Objective To observe the EGFR nuclear translocation in cervical carcinoma cell lines after irradiation and its possible role in radiation tolerance. Methods Western blotting was used to detect the nuclear EGFR and cytoplastic EGFR after irradiation. The effect of Cetuximab on expression of nuclear EGFR and survival fractions were investigated. Results After irradiation, compared with control group, the expression of nuclear EGFR protein increased in irradiated cervical carcinoma cell. Cetuximab inhibited the radiation-induced nuclear EGFR expression with decreased survival fractions. Conclusion Radiation could induce EGFR nuclear translocation in cervical carcinoma cell lines and nuclear EGFR might be correlated with radiation tolerance in Cervical carcinoma cell.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2009年第2期133-135,共3页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金（30672426）
