摘要
目的观察苯丙氨酸二肽类化合物073对2.2.15细胞分泌HBsAg、HBeAg的影响。方法以2.2.15细胞为研究对象,通过细胞毒性实验确定苯丙氨酸二肽类化合物073对细胞的最大无毒浓度(TC0),在最大无毒浓度以下将药物用培养液对倍稀释成50、25、12.5μg.mL-1加入2.2.15细胞进行培养,每4d换同浓度药物培养液;分别收集第4、8天以及停药后4天的培养液上清,酶联免疫法(ELISA)测定HBsAg、HBeAg含量。结果苯丙氨酸二肽类化合物073最大无毒浓度为50μg.mL-1,在最大无毒浓度时对HBsAg有明显的抑制作用,第8天时的抑制率为59.3%,半数抑制浓度(IC50)为22.9μg.mL-1,治疗指数(TI)为3.1;但对HBeAg无明显抑制作用,第8天时的抑制率仅为25.5%。结论苯丙氨酸二肽类化合物073对2.2.15细胞HBsAg有显著的抑制作用。
Aim To study the effect of phenylalanine dipeptide compounds 073 （ PDC073 ） on the secretion of HBsAg and HBeAg by 2.2.15 cells. Methods 2.2.15 cells were studied in the vitro model, and the maximum non-toxic concentration （ TC0 ） was identified by cell toxicity experiment of PDC073. Under the TC0, a pharmaceutical anti-virus experiment was performed. PDC073 was diluted by culture medium into different concentrations of 50, 25 and 12.5 μg·mL^-1 , which were added into the cell culture medium respectively. The medium old the same concentration was replaced every 4 days. On the 8th day the medium was changed to the drug-blank medium. The superuatant lipuid was collected on the 4th, 8th day and 4 days after treatment. ELISA method was used to assay the content of HBsAg and HBeAg. Results The maximum non-toxic concentration of PDC073 was 50μg·mL^-1. At this concentration of PDC073 the HBsAg was obviously IC50 was 22.9μg·mL^-1 and TI was 3.1. However, the inhibited. The inhibition ratio on the 8th day was 59.3% ; HBeAg was little inhibited, as the inhibition ratio was only 25.5% on the 8th day. Conclusion The PDC073 has significant inhibitive effect of HBsAg on cultured cell line 2.2. 15.
出处
《解放军药学学报》
CAS
2009年第2期120-124,共5页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家科技部"国际合作"新药基金项目
No.2008003
国家自然科学基金项目
No.30760292
贵州省中药现代化项目
黔科合农字[2006]5001号
973计划前期研究专项
No.2007CB516800
