摘要
目的:观察甲状腺功能减退症(简称甲减)大鼠心肌改变,及中药复方对其疗效。方法:(1)造模方法:选用清洁级健康Wistar大鼠70只,雌雄各半。取20只作为正常组:正常饲养。其余50只大鼠每天饲以实验专用的低碘饲料,同时喂1%高氯酸钠19天停用,续喂双蒸馏水2天。(2)分组:造模成功后随机分成4组:模型组、补中益气汤组、逍遥散组和金匮肾气丸组。(3)处理因素:模型组:低碘饲料,4mL双蒸水分两次等量灌服。补中益气汤组:低碘饲料,灌服4mL含生药量约为18.9g/kg的补中益气汤。逍遥散组:低碘饲料,灌服4mL含生药量约为14.9g/kg的逍遥散汤剂。金匮肾气丸组:低碘饲料,灌服4mL含生药量约为24.4g/kg的金匮肾气丸汤剂。观察各组大鼠一般情况,甲状腺激素及TSH水平,心肌酶谱CK及CK-MB的变化;心肌细胞形态的改变。结果:90天后,模型组血清CK(917.1±122.8)、CK-MB(903.7±165.4)明显高于正常组CK(483.8±178.6)、CK-MB(406.7±204.9),P均<0.01;补中益气汤组CK(583.9±172.5)、CK-MB(565.2±152.2)低于模型组、逍遥散组CK(662.5±227.1)、CK-MB(679.1±263.7),金匮肾气丸组CK(625.2±126.3)、CK-MB(630.2±128.3),明显高于正常组(P均<0.05)。逍遥散组,金匮肾气丸组,低于模型组比,P均<0.05。结论:在低碘致甲状腺功能减退机体中,与疏肝、温肾组相比健脾组可明显改善甲减大鼠的心肌细胞形态及心肌酶谱。
Objective：To observe cardiac muscle change in the iodine deficiency hypothyroid rats and the curative effect of Traditional Chinese Medicine. Methods ： By giving low iodine diet and 1% higher sodium chlorate for 19 days, and then feeding double-distilled water（DDW） for 2 days,the model rats were established. Then,the hypothyroid rats were randomly divided into 4 groups, the model, Xiaoyaosan group,Jingkuishenqi group and Buzhongyiqi Decoction group. The normal rats were given the common diet and DDW. The rats in other groups were given continuously low iodine diet and DDW. Results ：The model rats were shown as the symptoms of gradual emaciation, laziness, loose stools with occasional dry stools from the 3rd day of the experiment and reduced appetite and reduction of drinking water at the final stage of the experiment. The anus temperature of the model rats did not changed obviously. The cardiac muscle cells got degenerated and shrunk. In Buzhongyiqi Decoction rats, myocardial degeneration disappeared, the shrunk of cardiac muscle cells was significantly improved. But the rats in Xiaoyaosan group and Jinkuishenqi group did not improve. The values of serum CK （ 917. 1 ± 122. 8）, CK - MB（ 903. 7 ± 165. 4） of the model group were markedly higherly than those of the normal controls group[ CK（483.8 ± 178. 6） ,CK - MB （483.8 ± 178.6） ] ,both P 〈 0. 05. When compared with the model controls,the values of the serum CK（ 583.9 ± 172. 5 ） , CK - MB （ 565.2 ± 152. 2 ） of Buzhongyiqi Decoction group were markedly reduced,P 〈0. 05 ,compared with the control group,the values of Xiaoyaosan group[ CK（662. 5 ±227. 1 ） ,CK - MB（679. 1 ±263. 7） ]and Jingkuishenqi group[ CK（625. 2 ±126.3）, CK - MB （630. 2 ± 128. 3） ] were markedly imrpoved,P 〈 0. 05. Conclusion ： Buzhongyiqi Decoction can markedly improve the symptoms, the morphology of cardiac muscle ceils and cardiac muscle enzyme spectrum in the iodine deficiency hypothyroid rats and superior to other Chinese herbs
出处
《中华中医药学刊》
CAS
2009年第3期614-617,共4页
Chinese Archives of Traditional Chinese Medicine
基金
刊辽宁省教育厅资助项目(202153326)
辽宁省科技厅博士基金资助项目(20031039)
关键词
甲状腺功能减退
心肌
中药复方
iodine deficiency hypothyroid
cardiac muscle
chinese medicine
