摘要
目的:观察补肾丹对衰老模型大鼠的学习记忆能力的影响。方法:将清洁级健康Wistar雄性大鼠,随机分为7组:正常对照组、金匮肾气丸组、补肾丹高、中、低剂量组、维生素E组、模型组。除正常对照组外各组腹腔注射D-gal(250mg·kg-1.d-1)60天,采用D-半乳糖致亚急性衰老法[1],造成大鼠拟衰老动物模型。同时,正常对照组和模型对照组给等体积的生理盐水,金匮肾气丸组同时给予金匮肾气丸(0.72g.kg-1.d-1)灌胃,补肾丹高、中、低剂量组同时分别给予相当于生药2.7、1.35、0.675g.kg-1.d-1灌胃。维生素E组每日灌服维生素E0.027g/kg,每日1次,共60天。各组大鼠分别进行水迷宫法、跳台法实验,比较各组动物潜伏期和正确反应次数。结果:补肾丹高、中、低剂量组动物行为学指标明显改善,与模型组比较差异有统计学意义(P<0.05)。结论:补肾丹能够显著改善大鼠的学习记忆能力。
Objective：effect of Bushen Bolus on learning and memory capacity of aging rats. Methods：Wistar male rats of clean grade were randomly assigned into 7 groups： control group, Golden Cabinet Kidney Qi Bolus group, Bushen Bolus high,middle,low dosages groups,Vitamin E group,and model group. All groups except control group use intraperitoneal injection of D - gal（250mg.kg^-1.d^-1 ）for 60 days. The subacute senile rats model was established by injecting D - galactode. The control group and model group were given same volume of normal saline. Golden Cabinet Kidney Qi Bolus group was given Golden Cabinet Kidney Qi Bolus （0. 72g.kg^-1.d^-1） by mouth. Bushen Bolus high, middle,low dosages groups were given medicine 2.7g.kg^-1.d^-1,1.35g.kg^-1.d^-1 ,0. 675g.kg^-1.d^-1 by mouth. Vitamin E group was given Vitamin E 0. 027g/kg once a day for 60 days. We adopted Morris water maze and step down test to compare the del- itescence and right numbers of all the rats. Results ： The behavior indexes of Bushen Bolus high, middle,low dosages groups improve obviously. With the model group, the difference was statistically significant（ P 〈 0. 05 ）. Conclusion： Bushen Bolus can improve the rat＇s ability of learning and memory.
出处
《中华中医药学刊》
CAS
2009年第3期469-471,共3页
Chinese Archives of Traditional Chinese Medicine
关键词
补肾丹
学习记忆障碍
实验研究
Bushen bolus
learning and memory obstacle
experiment
