摘要
用脂多糖(LPS)诱导小鼠单核巨噬细胞RAW 264.7释放一氧化氮(NO),Griess法测定培养上清中NO释放量并计算抑制率,MTT法测定细胞存活率评价药物的细胞毒性,以检测腺梗豨莶不同溶剂萃取部位对活化巨噬细胞释放炎症介质NO的抑制作用.结果表明,石油醚及乙酸乙酯部位对活化巨噬细胞释放NO的抑制作用明显强于正丁醇部位及水层,即石油醚及乙酸乙酯部位为腺梗豨莶抑制活化巨噬细胞释放NO的主要活性部位,半数抑制浓度(IC50)值分别为6.0和6.5μg.mL-1,其作用强度优于该植物的主要活性成分奇壬醇(IC50为37.5μg.mL-1),推断该活性部位中应含有奇壬醇以外的其他活性化合物.以上结果为进一步阐明腺梗豨莶的抗炎作用物质基础提供了一定的理论基础.
To study the inhibitory effect of the extract from Siegesbeckia pubescens on the NO production in LPS-activated maerophage,the production of NO is induced by LPS and determine by Griess reaction,the inhibitory rate on the NO production is calculated and the cell cytotoxieity is evaluated by MTT method. The fractions of petroleum ether and ethyl acetate of S. pubescens show stronger inhibitory activity on NO production than the n-butanol and water fractions. The IC50 values of petroleum ether and ethyl acetate fractions are 6. 0 and 6. 5μg·mL^-1, respectively, which indicates the stronger activity than the main active constituent kirenol （IC50 = 37.54μg·mL^-1）. It seems that there are unknown active constituents in the above active principles. These findings might be valuable for further elucidation of the active constituents of S. pubescens.
出处
《烟台大学学报（自然科学与工程版）》
CAS
北大核心
2009年第2期137-140,共4页
Journal of Yantai University(Natural Science and Engineering Edition)
基金
烟台大学博士启动基金资助项目(YS06B4)
