摘要
目的了解医院近两年大肠埃希菌临床标本分离株的耐药性。方法采用K—B法检测435株大肠埃希菌对19种抗菌药物的耐药性，用三维试验检测AmpC酶和ESBLs。结果宫颈分泌物、尿液、咽拭子和恶露是检出数最高的标本；大肠埃希菌对氨苄西林、氨曲南、复方新诺明和喹诺酮类药物的耐药率位居前列（49．23％～84．10％），第三、四代头孢菌素的耐药率也高达31．35％～46．80％；尚未发现对亚胺培南和美罗培南耐药株，头孢哌酮／舒巴坦仍有93．38％的敏感率；ESBLs检出率32．67％，AmpC酶检出率5．52％，其中有19株为同时产ESBLs和AmpC酶，单独产AmpC酶菌6株。结论大肠埃希菌在临床标本中检出率和耐药率日趋严重，临床与实验室应共同采取应对措施，降低医院感染发生率，控制耐药菌的产生和播散。
OBJECTIVE To analyze the drug-resistance of Escherichia coli isolated from clinic in the past two years. METHODS The drug-resistance to 19 kinds of antimierobial agents of 435 E. coli strains was detected by K-B method. 3 D test was performed to detect AmpC β-lactamase and Extended-spectrum β-lactamases （ESBLs）. RESULTS It showed that the strains from cervical secretions, urine, throat swab and lochia specimens had the highest positive rate. Sensitivity test results showed that the resistance rate to ampicillin, aztreonam, sulfamethoxazole/trimethoprims and quinolones was high （49.23-84.10 %）, and resistance rate to the third, and fourth-generation cephalosporins was high too （31.35- 46. 80%）. Strains that resistant to imipenem and meropenem were not detected out and they had a high sensitivity rate （93. 38%）, to cefoperazone/sulbactam. Detection rate of ESBLs and AmpC was 32. 67% and 5.52%, respectively. Nineteen srtains produced both of them and 6 srtains produced AmpC only. CONCLUSIONS The detection rate and drug resistance are increasingly severe. Clinic and laboratory should make efforts to reduce hospital infection and dissemination of drug-resistant strains.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第8期992-994,共3页
Chinese Journal of Nosocomiology
关键词
大肠埃希菌
耐药性
超广谱Β-内酰胺酶
AMPC酶
Escherichia coli
Drug-resistance
Extended-spectrum β-lactamases
AmpC β-lactamase
