摘要
目的了解成人手足口病的病原体，并分析肠道病毒71型（EV71）核苷酸序列特征。方法RTPCR技术对4例成人手足口病患者标本进行肠道病毒基因检测，并测定EV71核苷酸序列，所得序列通过BLAST程序作EV71序列的进一步确认．与基因库中不同基因型EV71核苷酸序列进行比对分析、同源性分析，并构建种系进化树。结果4例患者肠道病毒通用引物和EV71特异性引物RT—PCR检测均阳性。经BLAST分析，测序所得4条核苷酸序列（分别命名为GZ19610、GZ99310、GZ99355和GZ46477）与EV71毒株序列同源。序列分析表明，4条核苷酸序列之间的同源性为96．0％～99．1％，与2008年初我国安徽省阜阳市暴发的手足口病疫情所分离的EV71毒株同源性最高。EV71序列遗传进化分析确定其均为EV71基因亚型C4，与阜阳、深圳、重庆、上海地区及2004年我国台湾地区流行毒株基因型一致．且距离最近。结论成人也可感染EV71引起手足口病，与我国现在及既往流行病毒株相比，此次4例成人手足口病患者感染的EV71未发生大的变异；应加强对成人手足口病患者的隔离治疗，以免其传播病毒引起疾病更大的流行。
Objective To identify the pathogens that cause hand, foot and mouth disease （HFMD） in adults and analyze the nucleotide sequences characteristics of enterovirus 71 （EV71）. Methods The reverse transcription-polymerase chain reaction （RT-PCR） method was used to detect the enterovirus from the samples of four adult HFMD patients. The 227 bp amplified segments of EV71 were then sequenced and compared with the sequences of previously isolated EV71 strains available from GenBank by homogeneity and phylogenetic tree analyses. Results All the results of RT PCR with enterovirus universal primers and EV71 specific primers were positive. The EV71 sequences analysis showed that the four new sequences （named as GZ19610, GZ99310, GZ99355 and GZ46477） shared 96. 0% to 99. 1% nucleotide identify themselves ahd shared 96. 90% to 100. 0% homology with the strain Fuyang/17.08/3 isolated in 2008 from Fuyang, Anhui Province. Phylogenetic tree analysis showed that the genotype of the four new sequences was all subtype C4, they were the same sub-genotype as those strains isolated from Chinese mainland and Chinese Taiwan in 2004, and the genetic distance between them was most closely. Conclusions EV71 can cause adult HFMD. Compared with the nucleotide sequences of EV71 strains that isolated now and formerly in China, there is no large variation of the EV71 sequences isolated from four adult HFMD patients in Guangzhou this time. The adult HFMD patients should be isolated for treatment to avoid them transmitting the virus and causing disease spreading.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2009年第3期156-160,共5页
Chinese Journal of Infectious Diseases
关键词
手足口病
肠道病毒属
基因型
成年人
碱基序列
反转录-聚合酶链反应
Hand, foot and mouth disease
Enterovirus
Genotype
Adult
Base sequence Reverse transcriptase polymerase chain reaction
