摘要
【目的】探讨弥散性神经内分泌细胞标记物在牛黄体细胞中的共存现象,以期为黄体细胞的内分泌研究提供理论依据。【方法】采集黄牛黄体,常规方法制备石蜡切片,采用免疫组织化学链霉素抗生物素蛋白-过氧化物酶法,结合免疫组化双标记法染色后,镜检拍照,研究牛周期性黄体细胞中5-HT与NSE和SYN的共存情况。【结果】黄体组织中含有这3种标记物的单标记和5-HT与其他两者的双标记免疫反应阳性细胞,其主要以散在形式分布于黄体中,细胞形态多呈圆形、椭圆形和梭形,阳性细胞平均数由多至少依次为:5-HT、NSE、SYN/5-HT、SYN和NSE/5-HT;在黄体细胞以外的卵巢细胞中未观察到这3种物质的存在。【结论】牛黄体组织中存在5-HT、NSE、SYN 3种标记物和SYN/5-HT、NSE/5-HT双标记免疫反应阳性细胞,提示黄体组织在发生的同时也存在着部分细胞的神经内分泌分化。
[Objective] The study was done to probe into the existence of diffused cells label in corpus luteum （CL） to provide theoretical foundation for the study of neuroendocrine. [Method] Corpus luteum of bovine was collected, paraffin slice prepared with routine method and dyed with immunohistochemistry streptavidin-peroxidase（SP） method combining with double-labeled immunohistochemistry, then a photo- graph was taken after examining under the microscope,and the coexistence of 5-HT.NSE and SYN in cycling corpus luteum cells of bovine was studied. [Result] The results showed that cells which were single positive of 5-HT.NSE or SYN and double positive of NSE/5-HT or SYN/5-HT were localized in CL. They were mainly distributed in CL with dispersive form,and were mostly round,oval and triangle. The average number of immunoreactive cells in one section in descending order was 5-HT single positive cell,NSE single positive cell,SYN/5-HT double positive cell,SYN single positive cell and NSE/5-HT double positive cell. The existence of cells containing three antigens was not observed in the cell of ovary except the CL cells. [Conclusion] It showed that three labels and the phenomena of 5-HT coexisted with NSE or SYN were localized in CL,and suggested that neuroendocrine differentiation exist in the portion of CL cells at the same time of the CL formation.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第4期37-41,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(30170683)
陕西省自然科学基金项目(2006C130)
河南省乳品工程技术研究中心科研基金项目(2006001)
