摘要
Objective To study the effect of testosterone propionate (TP) on changes of a growth associated protein (GAP 43) in facial nucleus after right facial nerves impacted in rabbits. Methods Facial nerve impacted injury model was made with type BIM Ⅱ biologic impact machine in the right facial nerves of New Zealand rabbits. Both changes of GAP 43 such as immunohistochemistic neurons (GLIN) in the right facial nerve nucleus and the ultrastructure of facial nerve on 1st, 3rd, 7th, 14th and 21st day after injury were observed with streptavidin peroxidease (SP) immunohistochemistry staining and with electron microscopy respectively. Results GLIN in the facial nerve nucleus in the dam aged side began to increase at the 1st day and reached peak at the 14th day of injury, and to decrease gradually at the day of 21st. GLIN in TP treated group increased significantly compared with that of the control group. There was more new myelinogeny in facial nerves in the TP treated group observed under electronic microscope on the 14th day after injury when compared with that of the controls. Conclusion TP may help the injured facial nerves in repairing and regenerating by increasing the synthesis and the expression of GAP 43.
Objective To study the effect of testosterone propionate (TP) on changes of a growth associated protein (GAP 43) in facial nucleus after right facial nerves impacted in rabbits. Methods Facial nerve impacted injury model was made with type BIM Ⅱ biologic impact machine in the right facial nerves of New Zealand rabbits. Both changes of GAP 43 such as immunohistochemistic neurons (GLIN) in the right facial nerve nucleus and the ultrastructure of facial nerve on 1st, 3rd, 7th, 14th and 21st day after injury were observed with streptavidin peroxidease (SP) immunohistochemistry staining and with electron microscopy respectively. Results GLIN in the facial nerve nucleus in the dam aged side began to increase at the 1st day and reached peak at the 14th day of injury, and to decrease gradually at the day of 21st. GLIN in TP treated group increased significantly compared with that of the control group. There was more new myelinogeny in facial nerves in the TP treated group observed under electronic microscope on the 14th day after injury when compared with that of the controls. Conclusion TP may help the injured facial nerves in repairing and regenerating by increasing the synthesis and the expression of GAP 43.
