摘要
Abstract Objectives To identify the effects of tuberculin purified protein derivative (PPD) sensitization on attenuating pulmonary T helper 2 (Th2) reaction and eosinophil infiltration in ovalbumin sensitized mice, and to search for the possibility of its clinical use in the management of asthma in a new way. Methods Sixty C57BL/6 mice were sensitized with PPD and then with ovalbumin and aluminum hydroxide, and randomized into 4 groups: ovalbumin (OVA), pre PPD, post PPD and control groups. Aerosol PPD were administered 3 h before or after ovalbumin challenge in the pre PPD and post PPD groups respectively, and control group received aerosol PPD only. IL 4, IL 5 expression was detected by immunocytochemistry in situ hybridization. Lung slides were stained with eosin and hemotoxylin, and pathological changes were observed. Results Ovalbumin aerosol inhalation caused a mixed inflammatory infiltration dominated by CD4 + T lymphocytes and eosinophils in the lung of sensitized mice. 87.5%-89.7% and 89.0%-89.2% of the CD4 + T lymphocytes were IL 4 mRNA + and IL 5 mRNA + respectively. 88.7%-91.2% of IL 4 mRNA + cells and 89.8%-90.6% of IL 5 mRNA + cells were CD4 + T lymphocytes in OVA group. Aerosol administration of PPD markedly suppressed IL 4 and IL 5 expression, and lung eosinophil infiltration. It was more effective in pre PPD group. 76.6%-78.0% of IL 4 mRNA + and 73.8%-79.7% of IL 5 mRNA + cells were CD4 + and 78.1%-84.9% and 78.4%-85.3% of the CD4 + cells were IL 4 mRNA + or IL 5 mRNA + respectively in pre PPD group, both were markedly lower than that of OVA group. CD4 + percentage of IL 4 mRNA + and IL 5 mRNA + cells were 80.7%- 82.0% and 78.0%-83.9% in post PPD group, which were markedly lower than that of OVA group. Conclusions Sensitization with PPD by intraperitoneal injection and then challenged by PPD inhalation markedly suppressed IL 4, IL 5 expression and eosinophil infiltration, and attenuated pulmonary Th2 reaction in ovalbumin sensitiz
Objectives To identify the effects of tuberculin purified protein derivative (PPD) sensitization on attenuating pulmonary T helper 2 (Th2) reaction and eosinophil infiltration in ovalbumin sensitized mice, and to search for the possibility of its clinical use in the management of asthma in a new way. Methods Sixty C57BL/6 mice were sensitized with PPD and then with ovalbumin and aluminum hydroxide, and randomized into 4 groups: ovalbumin (OVA), pre PPD, post PPD and control groups. Aerosol PPD were administered 3 h before or after ovalbumin challenge in the pre PPD and post PPD groups respectively, and control group received aerosol PPD only. IL 4, IL 5 expression was detected by immunocytochemistry in situ hybridization. Lung slides were stained with eosin and hemotoxylin, and pathological changes were observed. Results Ovalbumin aerosol inhalation caused a mixed inflammatory infiltration dominated by CD4 + T lymphocytes and eosinophils in the lung of sensitized mice. 87.5%-89.7% and 89.0%-89.2% of the CD4 + T lymphocytes were IL 4 mRNA + and IL 5 mRNA + respectively. 88.7%-91.2% of IL 4 mRNA + cells and 89.8%-90.6% of IL 5 mRNA + cells were CD4 + T lymphocytes in OVA group. Aerosol administration of PPD markedly suppressed IL 4 and IL 5 expression, and lung eosinophil infiltration. It was more effective in pre PPD group. 76.6%-78.0% of IL 4 mRNA + and 73.8%-79.7% of IL 5 mRNA + cells were CD4 + and 78.1%-84.9% and 78.4%-85.3% of the CD4 + cells were IL 4 mRNA + or IL 5 mRNA + respectively in pre PPD group, both were markedly lower than that of OVA group. CD4 + percentage of IL 4 mRNA + and IL 5 mRNA + cells were 80.7%- 82.0% and 78.0%-83.9% in post PPD group, which were markedly lower than that of OVA group. Conclusions Sensitization with PPD by intraperitoneal injection and then challenged by PPD inhalation markedly suppressed IL 4, IL 5 expression and eosinophil infiltration, and attenuated pulmonary Th2 reaction in ovalbumin sensitized mice.
