摘要
目的：探讨ｒｈＧＨ、ＩＧＦ－１对严重烧伤小鼠早期腹腔巨噬细胞（Ｍφ）ＣＤ１４ｍＲＮＡ转录的影响。方法：在体实验：２０只小鼠被随机地分成Ａ、Ｂ组（烧伤前连续５ｄ分别给予ｒｈＧＨ和生理盐水）和Ｃ、Ｄ组（假烫前连续５ｄ分别给予ｒｈＧＨ和生理盐水）。离体实验：１０只小鼠被分成Ｅ、Ｆ组（烧伤和假烫组），Ｍφ加入ｒｈＧＨ（０，１．６，８，４０ｎｇ／ｍｌ）和ＩＧＦ－１（０，０．２，１，５μｇ／ｍｌ）培养。用细胞原位杂交方法，观察了全身应用ｒｈＧＨ及体外培养加入ｒｈＧＨ、ＩＧＦ－１对小鼠巨噬细胞ＣＤ１４ｍＲＮＡ转录的影响。结果：在体实验：（１）预先肌注ｒｈＧＨ，可使假烫组小鼠腹腔ＭφＣＤ１４ｍＲＮＡ的转录明显增加。（２）烧伤后腹腔ＭφＣＤ１４ｍＲ－ＮＡ的转录也明显增加，预先应用ｒｈＧＨ的小鼠烧伤后不会出现ＭφＣＤ１４ｍＲＮＡ的过度转录。（３）体外培养实验：ｒｈＧＨ（４０ｎｇ／ｍｌ）可使烧伤小鼠ＭφＣＤ１４ｍＲＮＡ转录增加，其他浓度无显著改变，ＩＧＦ－１各浓度均增加ＣＤ１４ｍＲＮＡ的转录。结论：ｒｈＧＨ、ＩＧＦ－１可能通过增加ＭφＣＤ１４基因表达使Ｍφ易于被激活，从而有利于免疫功能的增强。ｒｈＧＨ的这一作用可能通过ＩＧＦ－１?
Objective: To study the effects of rhGH and IGF-1 on CD14 mRNA in peritoneal macrophages in severely burned rats. Methods: In vivo: Twenty mice were randomly divided into burn group(given rhGH or NS for 5 d before burned) and sham group(given rhGH or NS). In vitro: Mouse peritoneal macrophages from burn group (n=5) or sham group (n=5) were isolated and incubated with rhGH (0,1.6,8,40 ng/ml) or IGF1 (0,0.2,1,5 μg/ml). The peritoneal macrophages CD14 mRNA expression were observed by in situ hybridization analysis. Results: Significantly increased CD14 mRNA staining granules in cytoplasm were found in burn mice (NS or rhGH) and sham group(rhGH). No difference was found between macrophages from burned mice treated with rhGH and those from burned mice injected with NS. The CD14 mRNA levels in the cells from sham or burn group incubated with all doses of IGF-1 were elevated. These changes were also observed in the cells incubated with rhGH from burn group (all doses) and from sham group (40 ng/ml).Conclusion: rhGH and IGF-1 significantly enhances macrophage sensitivity possibly because of CD14 mRNA expression changes. The increase is also seen in burn mice and could not be further enhanced by rhGH.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1998年第S1期95-97,共3页
Academic Journal of Second Military Medical University
