A New Competitive Inhibitory Monoclonal Antibody to Carboxypcptidase A(CPA)

A New Competitive Inhibitory Monoclonal Antibody to Carboxypcptidase A (CPA)

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摘要 The monoclonal antibody ID11D7 to carboxypeptidase A (CPA) was prepared. Theinhibitory effect of McAb ID11D7 on the peptidase activity of CPA was measured. The result reflectsthat the McAb ID11D7 can competitively inhibit the peptidase activity of CPA with an apparentinhibitory constant of 6.3 × 10-9M. Based on this work. the McAb ID11D7 can be used as idiotypicantigen to prepare anti-idiotypic catalytic antibody with the peptidase activity similar to CPA. The monoclonal antibody ID11D7 to carboxypeptidase A (CPA) was prepared. Theinhibitory effect of McAb ID11D7 on the peptidase activity of CPA was measured. The result reflectsthat the McAb ID11D7 can competitively inhibit the peptidase activity of CPA with an apparentinhibitory constant of 6.3 × 10-9M. Based on this work. the McAb ID11D7 can be used as idiotypicantigen to prepare anti-idiotypic catalytic antibody with the peptidase activity similar to CPA.

作者 Mei DU Sheng JIN Zhen Quan GUO(College of Chemistry and Molecular Engineering. Peking University. 100871)(College of Life Science, Peking University, 100871)

出处《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第2期121-122,共2页 中国化学快报（英文版）

关键词 Monoclonal antibody carboxypeptidase A (CPA) competitive inhibition peptidaseactivity Monoclonal antibody, carboxypeptidase A (CPA), competitive inhibition, peptidaseactivity

分类号 O629 [理学—有机化学]

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Chinese Chemical Letters

1999年第2期

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A New Competitive Inhibitory Monoclonal Antibody to Carboxypcptidase A(CPA)

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