摘要
体外实验证明１０μｇ．ｍｌ－１去甲斑蝥素作用于人红白血病Ｋ５６２细胞２４ｈ，可诱导Ｋ５６２细胞发生凋亡。提取加药组细胞ＤＮＡ进行琼脂糖凝胶电泳呈现典型的ＤＮＡ“梯子”，同时加药组细胞出现核固缩、碎裂及胞浆浓缩等形态学变化。流式细胞测试结合形态学观察，特别是细胞超微结构观察，证明去甲斑蝥素诱导的Ｋ５６２细胞凋亡大部分发生在细胞周期的Ｍ期，也有部分发生在间期。免疫细胞化学结果表明，１０μｇ．ｍｌ－１去甲斑蝥素作用于人红白血病Ｋ５６２细胞２４ｈ，与对照组相比，加药组细胞中ｂｃｌ┐２蛋白表达增强，而Ｂａｘ蛋白表达减弱，两组间有显著性差异（Ｐ＜０．００１）。
It's proved that 10μg/ml -1 NCTD can induce apoptosis in K562
human myeloid leukemia cells in vitro after 24h. A characteristic DNA 'ladder' was detected by
using agarose gel electrophoresis. Some treated K562 cells were found condensed with nuclear
chromatin compaction and breaking up. By FACS analysis and morphologial observation,
especially by ultrastructural observation, we proved that most apoptosis of K562 cells occurred
in mitosis phase and some others initiated in interphase of cell cycle. It's found the expression
of bcl 2 oncoprotein was decreased while Bax increased in the treated K562 cells by
immunocytochemical staining, which was in striking contrast with the control group (P<0 001).
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1999年第1期93-99,共7页
Chinese Journal of Histochemistry and Cytochemistry
基金
高等学校博士学科点专项科研基金
