摘要
应用原位杂交技术和免疫组织化学法对８例Ｂ淋巴瘤和４例Ｔ淋巴瘤进行了ＩｇＨｍＲＮＡ和ＩｇＭ，κ，λ的检测。结果显示，８例Ｂ淋巴瘤ＩｇＨｍＲＮＡ均为阳性，位于胞核周围，有的ｍＲＮＡ阳性反应成点状位于胞质的一侧。７例胞质中ＩｇＭ，κ或λ为阳性。１例胞质内无ＩｇＭ，κ或λ，但胞核内ＩｇＨｍＲＮＡ阳性反应成点状分布，可能是初级ＲＮＡ转录。在Ｔ淋巴瘤中，只有一例胞核内出现初级ＲＮＡ转录，因为早期Ｔ淋巴瘤，也有Ｄ。
In this study, in situ hybridization and immunocytochemistry were used to detect IgHmRNA,
IgM, Kappa or Lambda in T and B lymphoma. The results indicated that in 8 cases of B
lymphomas IgHmRNA was positive, found in perinuclear region and also frequently in one side
of the cytoplasm. Simultanously, these cells were reactive with IgM, Kappa or Lambda. One B
lymphoma was completely lack of IgM, Kappa or Lambda, but the IgHmRNA appeared as
particles located in the nucleus, which might be primary RNA transcript. In T lymphomas
primary RNA transcript was found only in the cell nucleus of one case, because T lymphoma
also contained D, J rearrangement in the early stage of mutation.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1999年第1期20-23,共4页
Chinese Journal of Histochemistry and Cytochemistry
基金
福建省自然科学基金
关键词
免疫球蛋白重链mRNA
淋巴瘤
基因重排
原位杂交
Immunoglobulin
heavy chain mRNA
\ Lymphoma
\ Gene rearrangment
In situ Hybridization
