摘要
To investigate the expression of interleukin 2 (IL 2), metabotropic glutamate receptor subunit 1 (mGluR1) and estrogen receptor (ER) in neurons of the rat central nervous system (CNS) and identify the coexistence possibility of these immune neuro endocrine substances in the central neurons, the tri labeling immunocytochemical technique with different species specific primary antibodies (goat anti IL 2 antibody, rabbit anti mGluR1 antibody and mouse anti ER antibody ) were used to incubate two serial neighbor sections (one for demonstrating IL 2, another for mGluR1 and ER) of the cerebral cortex, medulla oblongata and spinal cord. There were IL 2 , mGluR1 and ER immunoreactivity (IR) positive labeled neurons in the above mentioned central areas. The IL 2 IR production showed brown color, located in the cytoplasm; In the neighbor serial section, the mGluR1 IR, production showed blue black color, located on the cell membrane; the ER IR production also showed brown color, located in the cytoplasm and nuclei. There were mGluR1/ER double labeled cells in the same section, which accounted for about 50 %-60 % of the total single and double labeled neurons. It was identified by projection check of serial neighbor sections that had mGluR1/ER/IL 2 tri labeled cells, which accounted for about 30 % of total mGluR1/ER double labeled neurons. The results indicate that mGluR1, ER and Il 2 can coexist in the same rat central neurons, therefore, providing morphological basis for the theory about immune neuro endocrine network at the cellular level for the first time.
To investigate the expression of interleukin 2 (IL 2), metabotropic glutamate receptor subunit 1 (mGluR1) and estrogen receptor (ER) in neurons of the rat central nervous system (CNS) and identify the coexistence possibility of these immune neuro endocrine substances in the central neurons, the tri labeling immunocytochemical technique with different species specific primary antibodies (goat anti IL 2 antibody, rabbit anti mGluR1 antibody and mouse anti ER antibody ) were used to incubate two serial neighbor sections (one for demonstrating IL 2, another for mGluR1 and ER) of the cerebral cortex, medulla oblongata and spinal cord. There were IL 2 , mGluR1 and ER immunoreactivity (IR) positive labeled neurons in the above mentioned central areas. The IL 2 IR production showed brown color, located in the cytoplasm; In the neighbor serial section, the mGluR1 IR, production showed blue black color, located on the cell membrane; the ER IR production also showed brown color, located in the cytoplasm and nuclei. There were mGluR1/ER double labeled cells in the same section, which accounted for about 50 %-60 % of the total single and double labeled neurons. It was identified by projection check of serial neighbor sections that had mGluR1/ER/IL 2 tri labeled cells, which accounted for about 30 % of total mGluR1/ER double labeled neurons. The results indicate that mGluR1, ER and Il 2 can coexist in the same rat central neurons, therefore, providing morphological basis for the theory about immune neuro endocrine network at the cellular level for the first time.
