摘要
Objective To explore the biological features of basaloid squamous cell carcinoma (BSC) of the esophagus.Methods Cytokeratins (CK4, CK18 and CK19), epithelial membrane antigen (EMA), carcino embryoantigen (CEA), α-smooth muscle antigen (α-SMA), S-100, laminin (LN), collagen Ⅳ (Col-Ⅳ), neuralspecific enolase (NSE), proliferating cell nuclear antigen (PCNA) and p53 antibodies were used to detect the corresponding antigen expression in 8 cases of BSC with ABC immunohistochemical methods.Results Two kinds of BSC cell components have different responses to the above antibodies. For basaloid cells (BCs), 7 cases were positive for CK19, and were negative for the other 4 epithelial antibodies CK4, CK18, CEA and EMA. BCs of 4 cases were positive to the muscular antibodies α-SMA and S-100, and the hyaline degeneration in the tumor nests was positive for LN and Col-Ⅳ. BCs had a high index of PCNA, with an average level of 54%. For squamous cells (SCs), 7 cases were positive for the epithelial antigen CK4, CEA and EMA, but were negative for CK19, α-SMA and S-100. The index of PCNA of SC was low, with an average level of 25%.Conclusion BSC of the esophagus is a high-malignancy tumor which is of multi-oriented differentiation.BCs represent basal cells which have the tendency of myoepithelial differentiation and have strong proliferation ability, whereas SCs represent typical squamous cell differentiation.
目的 了解食管基底细胞样鳞状细胞癌 (BSC)的生物学特点。方法 食管基底细胞样鳞状细胞癌 8例 ,用CK4 ,CK18,CK19,EMA ,CEA ,α SMA ,S 10 0 ,LN ,Col Ⅳ ,NSE ,PCNA ,p5 3等 12种抗体进行免疫组化常规ABC法检测。结果 BSC的两种细胞成分 ,即基底样细胞 (BC)和鳞状细胞 (SC)对上述抗体反应截然不同。绝大部分病例 (7例 )BC专一地对CK19抗体呈阳性反应 ,而对其它 4种上皮性抗体呈阴性反应 ;有 4例BC对α SMA及S 10 0肌性抗体呈阳性 ;BC巢内玻璃样物对LN及Col Ⅳ呈阳性反应 ;BC有较高的PCNA指数 ,平均为 5 4 %。相反 ,7例SC成分均对CK4、CEA、EMA等上皮性抗体呈阳性 ;对CK19、α SMA、S 10 0呈阴性 ;PCNA指数较低 ,平均为 2 5 %。结论 食管BSC为多向分化的高度恶性肿瘤。BC代表向基底细胞和肌上皮方向分化 ,其增殖活性强 ;而SC代表向典型的鳞状细胞分化。
