摘要
Objective To investigate the distribution and clonality of the T-cell receptor (TCR) Vβ repertoire in chronic graft versus host disease (cGVHD).Methods The complementarity determining region 3 (CDR3) of the TCRβ gene with 24 variable regions was amplified in peripheral blood mononuclear cells drawn from one cGVHD patient after allogenic bone marrow transplantation (allo-BMT) 35, 39, 43 or 45 months respectively, using RT-PCR, to observe the expression of TCR Vβ repertoire T cells. The PCR products were further analyzed by genescan to evaluate clonality of T cells. Ressults Fourteen or 16 TCR Vβ subfamily T ceils were detected in each sample of cGVHD case. Oligoclonal T cells were identified in TCR Vβ 6, 16, 17, 19 and 21 subfamilies. The stable clonal T cells in all samples were identified in Vβ6, Vβ17 and Vβ21 subfamilies.Conclusion Skewing distribution and stable clonal expansion of T cells can be found in cGVHD cases and it may be related to the initiation of cGVHD.
目的 分析慢性移植物抗宿主病 (cGVHD)中TCRVβ谱系的分布和克隆性。方法 应用RT PCR扩增一例CML异基因骨髓移植后cGVHD病人第 35,39,4 3和 4 5个月的外周血单个核细胞的TCRVβ 2 4个亚家族的CDR3,了解病人TCRVβT细胞的表达 ,PCR产物进一步经基因扫描分析确定T细胞克隆性。结果 cGVHD病人每个标本存在 14 16个Vβ家族T细胞 ;寡克隆T细胞可见于Vβ6 ,16 ,17,19和 2 1亚家族 ,稳定性克隆性TCRVβ6、17和 2 1T细胞可见于全部标本。结论 cGVHD病人存在倾斜性分布和克隆性生长T细胞 。
