摘要
目的 :研究一氧化氮合酶 (NOS)在耳蜗核复合体 (CNC)及脑干的分布特点 ,探讨一氧化氮 (NO)在听觉径路中的作用 ;方法 :采用依赖还原型辅酶Ⅱ的尼克酰胺腺嘌呤二核苷酸磷酸黄递酶 (Nicotinamideadeninedinucleotidephosphate -Diaphorase ,NADPH -d)组织化学方法 ,研究了豚鼠听觉核团和脑干内NOS的分布 ;结果 :在脑桥的各级听觉传入核团 ,均有NOS阳性神经元 ,而上橄榄复合体NOS反应阴性 ;耳蜗核有NOS阳性神经元 ,其NOS阳性神经元主要集中在后腹核 ,为圆形或椭圆型双极细胞 ;下丘的臂内侧核NOS阳性神经元呈小的、圆形细胞。在脑干中 ,NOS阳性神经元分布广泛 ;在三叉神经中脑核 (Me5 )、蓝斑 (LC)、前庭内侧核(MVe)、MVe复侧核 (MVeV)、前庭外侧核 (Lve)、舌下神经前置核 (PrH)、巨细胞网状核 (Gi)、Gi腹侧部 (GiV)、外侧巨细胞旁核 (LPGi)、背侧巨细胞旁核 (DPGi)均有NOS阳性神经元 ;结论 :NO可能是听觉中枢的神经递质或调质 ,参与声信号传递的调节 ;并可能与脑干功能的调节有关。
Aim:In this experiment,Nicotinamide adenine dinucleotide phosphate-Diaphorase (NADPH-d) histochemistrymethod was adopted to study the distributive character of positive nitric oxide synthase(NOS) in cochlear nuclear complex(CNC)and potine as guinea pig and explore the role of nitric oxide (NO) in auditory pathway.The result showed that NOS positive neurons were found in every auditory afferent nucleus,and NOS negative neurons were found in the superior olivary nucleus.Most of NOS positive neurons which were round or oval bipolar neurons were located in the ventual conchlear nucleus (VCN).NOS posititive neurons were also found in potine widely,they are in:Mesencephalic nucleus of 5th nerve(Me5)?Locus ceruleus(LC)?Medial vestibular nucleus(MVe)?Ventral part of Mve(MVeV)?Lateral veatibular nucleus(LVe)?Prepositus hypoglossal nucleus(PrH)?Gigantocellular reticular nucleus(Gi)?Ventral part of Gi (GiV)?Lateral paragigantocellular nuclers(LPGi)?Dorsal paragigantocellular nucleus(DPGi).Conclusion:The results suggest that NOS likely to be a transmitter or mediatou involved in regulating the transmission of sound signals,and may play an important role in the potine.
出处
《医药论坛杂志》
2001年第13期1-3,66,共4页
Journal of Medical Forum
基金
河南省自然科学基金资助课题
关键词
一氧化氮合酶
听觉中枢
脑桥
豚鼠
nitric oxide synthase(NOS)
auditory centre
histochemical
guinea Pig
potine
