摘要
目的克服聚乳酸材料的疏水性,制备表面特性和孔隙结构均符合组织工程需要的细胞支架。方法应用改进的溶液浇注／粒子沥滤技术,制备孔隙完全连通的三维聚乳酸细胞支架,碱液预处理支架表面,浸润明胶溶液,戊二醛蒸汽交联支架上的明胶,获得杂化改性支架;用扫描电镜等对改性后的支架进行结构与性能表征。结果圆柱形多孔聚乳酸支架,在杂化改性后吸水率增加约2倍,由改性前的(1164．2±172．9)％上升到(2637．7±527．8)％,亲水性显著增强;杂化改性后,明胶均匀分布在支架孔隙表面,支架孔隙形态、连通性基本没有变化。结论明胶杂化能显著提高聚乳酸细胞支架的亲水性,改性过程不影响支架的孔隙结构和形态,可以制备出亲水性好、内部结构可控、孔隙率高、孔隙连通性好的组织工程细胞支架。
Objective To overcome the hydrophilia of poly (lactic acid) (PLA), fabricate 3D porous PLA scaffolds with controllable pore-structure and surface properties to meet the requirement of tissue engineering. Methods 3D porous PLA scaffolds with controllable pore-structure were generated by technique of modified solvent casting/particulate leaching. Treated the scaffolds in sodium hydroxide solution, immerged into gelatin solvents, cross-linked the gelatin on pore surface of scaffold with glutaraldehyde, then removal unreacted aldehyde groups with 5% sodium borohydride solution, washed scaffolds with distilled water and dried. Then the PLA /gelatin hybrid scaffold was harvested, and characterized by scanning electron microscope (SEM) as well as other methods. Results Column-like porous PLA/gelatin scaffolds with controllable inner-structure were generated. The SEM photos showed that the pore-structure of scaffold remained unchanged after gelatin hybrid, and the gelatin cover almost all the pore surfaces. The action of scaffolds in water revealed that the unmodified one kept on floating while modified one under water. Conclusion The hydrophilia of scaffolds was improved effectively by hybrid. 3D PLA scaffolds with controllable pore-structure combined with gelatin hybrid method might be a better technique for fabrication scaffolds with good hydrophilia as well as pore structure, and this kind of scaffolds might have potential application for tissue engineering.
出处
《组织工程与重建外科杂志》
2005年第5期283-286,共4页
Journal of Tissue Engineering and Reconstructive Surgery
关键词
三维细胞支架
明胶
杂化改性
亲水性
孔隙连通
Three-dimensional cells scaffolds
Gelatin
Modification with hybrid
Hydrophilia
Interconnection
