摘要
目的检测基质金属蟹白酶-1、3、9（MMP-1、3、9）在曝光部位和非曝光部位皮肤中的表达，探讨它们在皮肤光老化机制中的作用。方法应用免疫组化方法分别对23例女性健康志愿者前臂伸侧（曝光）和上臂内侧（非曝光部位）皮肤石蜡标本巾的MMP-1、MMP-3、MMP-9进行检测，表达强度以免疫反应强度分布指数（IRIDI）表示，采用Wilcoxon符号秩和检验、Mann—Whitney秩和检验及Spearman秩相关分析进行统计学处理。结果MMP-1、MMP-3、MMP-9存曝光和非曝光部位均表达，曝光部位皮肤组织中MMP～1、MMP-3、MMP-9的IRIDI均值（范围）分别为7．70（3～12）、9．22（6～12）、8．30（6～12），非曝光部位分别为4．26（2～6）、5．39（2～9）、4．04（1～6），曝光部位MMP-1、MMP-3、MMP-9的IRIDI均显著高于非曝光部位（均P〈0．01）。50岁以上组曝光部位皮肤组织MMP-1、MMP-3、MMP-9的IRID[分别为9．17（6～12）、10．58（8～12）、8．92（8～12），非曝光部位分别为4．75（2～6）、6．42（4～9）、4．33（3～6），曝光部位均显著高于非曝光部位（P〈O．05）；50岁以下组曝光部位皮肤组织MMP-1、MMP-3、MMP-9的IRIDI分别为6．09（3～8）、7．73（6～9）、7．64（6～12），非曝光部位分别为3．73（2～6）、4．27（2～8）、3．73f1～6），曝光部位均显著高于非曝光部位（P〈0．05）；50岁以上组曝光部位显著高于50岁以下组，而两组非曝光部位皮肤组织MMP-1、MMP-9的IRIDI值差异尤统计学意义（P〉0．05）。曝光部位皮肤组织MMP-1、MMP-3、MMP-9的IRIDI值与年龄呈正相关（r=0．66、0．69、0．74，P〈0．01），非曝光部位皮肤组织MMP-1、MMP-9的IRIDI值与年龄无相关性。结论MMP-1、MMP-3、MMP-9在曝光部位表达显著高于非曝光部位，三者在光老化的发生发展中均起作用，但作用机制可能不同。
Objective To study the expression of matrix metalloproteinase （MMP）-1, -3 and -9 in sun-exposed and -unexposed skin as well as its significance in the mechanism of skin photoaging. Methods Skin samples were resected from the extensor side of forearm （ sun-exposed area） and flexor side of upper arm （sun-unexposed area） of 23 healthy female volunteers. The expression of MMP-1, -3 and -9 was detected by immunohistochemical staining in 46 skin samples. Immunoreactive intensity distribution index （IRIDI） was calculated to assess the expression of MMP-1, -3 and -9. Wilcoxon signed ranks test, Mann-Whitney U-test and Spearman rank correlation analysis were performed. Results MMP-1, -3 and -9 were expressed in both sun-exposed and -unexposed skin. The average IRIDI value for MMP-1, -3 and -9 was 7.70 （range, 3 to 12）, 9.22 （range, 6 to 12）, 8.30 （range, 6 to 12） in sun-exposed skin, and 4.26 （range, 2 to 6）, 5.39 （range, 2 to 9）, 4.04 （range, 1 to 6） in sun-unexposed skin, respectively; significant difference existed between sun-exposed and -unexposed skin in the three parameters （all P 〈 0.01 ）. A significant increase was observed in the average IRIDI value for MMP-1, -3 and -9 in sun-exposed skin vs. sun-unexposed skin in women above 50 years of age （9.17 vs 4.75, 10.58 vs 6.42, 8.92 vs 4.33, respectively, all P 〈 0.05 ）. In women younger than 50 years, the average IRIDI value for MMP- 1, -3 and -9 was 6.09 （range, 3 to 8）, 7.73 （range, 6 to 9）, 7.64 （range, 6 to 12） in sun-exposed skin, significantly higher than that in sun-unexposed skin [3.73 （range, 2 to 6）, 4.27 （range, 2 to 8）, 3.73 （range, 1 to 6）, all P 〈 0.05]. Increased IRIDI scores of MMP-1, -3 and -9 were noticed in sun-exposed skin in women above 50 years of age vs. those younger than 50 years, but there was no statistical difference in MMP-1 or MMP-9 between the two aged groups in sun-unexposed skin（all P 〉 0.05 ）. The IRIDI scores of MMP-1, MMP-3 and MMP-9 were positively
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2009年第4期244-247,共4页
Chinese Journal of Dermatology
基金
基金项目：中央级公益性科研基金（IMBF20070602）志谢 本实验及图像分析、拍片过程中得到本院病理科桑红桂、李阿梅、邵雪宝老师及高莹、薛燕宁博士的热情帮助,在数据分析时得到流行病室李婧博士的帮助
