摘要
通过生物芯片实验,观察约氏疟原虫（P.yoelii）来源的巨噬细胞迁移抑制因子（Macrophage migration inhibitory factor,MIF）（PyMIF）和宿主小鼠来源的MIF分子（MuMIF）在调节宿主细胞信号通路方面的差异。亲和纯化带有His标签的重组PyMIF和MuMIF蛋白,并去除内毒素后,分别加入体外培养的RAW264.7细胞,10h后收集细胞提取RNA,进行芯片分析。PyMIF和MuMIF对Jak-STAT信号通路没有直接调节作用,但能够通过对MAPK等其他通路的调节,活化或抑制共有的下游分子。宿主小鼠巨噬细胞是PyMIF的靶细胞,PyMIF通过活化癌基因等下游信号分子维持靶细胞活性。
To compare the regulation difference of signal pathway in host cell between P. yoelii-derived maerophage migration inhibitory factor （PyMIF） and its homologues in mouse （MuMIF）, we affinity purified the recombinant PyMIF and MuMIF with His tag, added them into the culture of RAW264.7 after removing LPS, collected the cells after 10 hours and extracted total RNA with Trizol, then we performed the Real-time PCR chip experiment. The data were ultimately analyzed by statistical method. PyMIF and MuMIF didn＇t regulate the Jak-STAT signal pathway directly, but regulated other pathways such as MAPK pathway, by either activated or inhibited the same downstream signal factor. We found that PyMIF acted on the macrophages of mouse to maintain the activity of the target cells by up-regulating oncogenes along the down stream of the signal pathways.
出处
《寄生虫与医学昆虫学报》
CAS
2009年第1期1-5,共5页
Acta Parasitologica et Medica Entomologica Sinica
