摘要
目的:探讨小檗碱预防颈动脉粥样硬化形成的作用机制。方法:将24只雄性新西兰大白兔随机分为对照组、模型组和小檗碱组各8只。对照组给予普通饮食;模型组和小檗碱组给予高脂饲料喂养1周后行右侧颈动脉内膜空气干燥术,术后,前者继续高脂饲料喂养4周以制成颈动脉粥样硬化模型,后者在前者的基础上每日灌服小檗碱(100 mg/kg)。第5周麻醉处死,取右侧颈动脉组织,行HE染色观察颈动脉病理改变,采用RT-PCR法测定VCAM-1 mRNA含量。结果:与对照组比较,模型组颈动脉内膜明显增生,内膜下可见大量泡沫细胞堆积,有明显粥样硬化斑块形成;而小檗碱组内膜轻度增厚,内膜下仅有少量泡沫细胞。模型组、小檗碱组和对照组VCAM-1/-βactin扩增带吸光度比值依次降低,差异有统计学意义(0.61±0.11,0.20±0.04,0.81±0.11,P<0.01)。结论:小檗碱可能通过抑制VCAM-1的表达预防颈动脉粥样硬化的形成。
Objective： To investigate the mechanisms of berberine of preventing atherosclerosis （AS） in rabbits. Methods：New Zealand white rabbits （n=24） were randomly divided into control, carotid artery atherosclerosis and berberine treatment groups （n=8 for each）. The rabbits of the control group were fed with normal diets. Carotid atherosclerosis was established by an air-drying operation performed on the right common carotids. The rats with carotid artery atherosclerosis received a high cholesterol-diet at the second week for 4 weeks. Berberine was administrated through a gastric tube daily starting from the first week for 5 weeks. The right carotid arteries were checked by Hematoxylin and Eosin （HE） staining to investigate the effects of berberine on the atherosclerotic lesions. RT-PCR was applied to study the expression of the vascular cell adhesion molecule-1 （VCAM-1）. Results：The air-operation in conjunction with a high cholesterol diet have generated a significant increase of foam cells when compared with those in the control group, which were inhibited by berberine treatment. The relative expression of VCAM1 mRNA was also increased significantly after induction of carotid artery atherosclerosis （P〈0.01）. In addition, Berberine administration had suppressed the enhancement of VCAM-lmRNA. Conclusion： Berberine could partially prevent the formation of atherosclerosis on the carotid artery walls by inhibiting the expression of VCAM-1.
出处
《神经损伤与功能重建》
2009年第2期102-104,共3页
Neural Injury and Functional Reconstruction
