摘要
本研究选用１月龄小鼠，观察了海马内注射ＣａＣｌ２造成高钙水平对小鼠学习和记忆行为的影响，并对海马ＣＡ３区进行突触超微结构定量分析。结果表明，与对照组相比：（１）ＣａＣｌ２组小鼠一次性被动回避反应的步入潜伏期有缩短的趋势，说明该组小鼠记忆减弱；同时Ｙ－迷宫分辨学习能力也显著下降（Ｐ＜０．０１）；（２）ＣａＣｌ２组海马ＣＡ３区突触后致密物质极显著变薄（Ｐ＜０．００１），而突触间隙宽度显著增大（Ｐ＜０．０５）。结果提示，海马突触界面结构参数的变化可能是Ｃａ２＋浓度过高损害学习记忆功能的形态结构基础。
お? In this study,the effect of high Ca2+ level by injecting CaCl2 into bilateral hippo-campus on the learning and memory behavior of young mice was investigated,and the synaptic structural parameters in the hippocampal CA3 area were quantitatively analysed.The main results were as follow,compared with control:(1) The STL(step-through latency)of CaCl2 group was shortened in one trial passive avoidance response,and the descrimination learning capacity of CaCl2 group in Y-maze was greatly attenuated(P<0.01),showing the severe impairment of learning and memory in this group. (2) The thickness of post-synaptic density(PSD)of CaCl2 group was thinned signi-ficantly(P<0.001),while the width of synaptic cleft of this group was apparently increased(P<0.05). These results suggested that the learning and memory dysfunction caused by over-loaded calcium ions in hippocampus might based on the morphological changes of the synaptic interface stucture.
基金
国家自然科学基金
关键词
突触界面结构
电镜
学习记忆障碍
高钙症
Synaptic interface structure,Learning-memory,Electron microscopy,Calcium ions
