摘要
采用70%乙醇渗漉提取,正丁醇萃取,常压硅胶柱反复层析,结合杀鼠活性追踪的方法,从豆科植物皂荚（GleditsiasinensisLam.）的果实中分离到一个含有7个糖基的三萜皂苷,通过TLC、mp、IR、MS、1H-NMR和13C-NMR等方法鉴定为3-O-β-D-吡喃木糖基-（1→2）-α-L-吡喃阿拉伯糖基-（1→6）-β-D-吡喃葡萄糖基齐墩果酸28-O-β-D-吡喃木糖基-（1→3）-β-D-吡喃木糖基-（1→4）-α-L-吡喃鼠李糖基-（1→2）-[（6S,2E）-6-羟基-2,6-二甲基-2,7-辛二烯醇-（1→6）]-β-D-吡南葡萄糖酯;对小鼠的毒性测定表明,试鼠经口灌胃的耐受量为200-800 mg/kg,LD50为508.31 mg/kg,95%可信区间为449.40-574.95 mg/kg.该三萜皂苷为皂荚杀鼠的活性成分.
A triterpenoid saponin was isolated from the pod of Gleditsia sinensis Lam. by 70% ethanol extraction and silica gel column chromatography, and was identified by TLC, nap, IR, MS, ^1 H-NMR and ^13 C- NMR. Its structure was determined to be 3-O-β-D-xylopyranosyl （1→2）-α-L-arabinopyranosyl-（1→6）-β-D- glucopyranosyl oleanolic acid 28-O-β-D-xylopyranosyl-（ 1 → 3 ）-β-D-xylopyranosyl-（ 1 →4 ）-α-L-rhamnopyr- anosyl-（1→ 2 ）-[- （ 6S, 2E）-f-hydroxy-2,6 dimethy 2,7-octadienoyl-（ 1→ 6 ）-β-D-glucopyranosyl ester. The acute toxicity experiment indicated that the tolerance dose was 2004800 mg/kg through mouth,and LD50 was 508.31mg/kg,with the 95% confidence limit of 449.40 mg/kg-574.95 mg/kg. This triterpenoid saponin was a raticide component of G. sinensis Lam..
出处
《西北植物学报》
CAS
CSCD
北大核心
2009年第3期618-621,共4页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家"十五"科技支撑项目(2006BAD08A1004)
国家林业局重点项目(2006-49)
林业公益性行业科研专项(200704012)
陕西省自然科学基金(2005C121)
关键词
皂荚
杀鼠活性
活性成分
急性毒性
Gleditsia sinensis Lam.
raticide acticity
active component
acute toxicity
