摘要
目的通过研究脑室注射β-淀粉样蛋白(Aβ)单体后的老年大鼠异氟醚(I)、七氟醚(S)或异丙酚(P)麻醉对Morris水迷宫训练成绩的影响。方法100只20个月龄的雄性老年大鼠分为对照组、Aβ1组、Aβ2组、Aβ3组、I+生理盐水(NS)组、I+Aβ1组、S+NS组、S+Aβ1组、P+NS组、P+Aβ1组。麻醉后2周进行Mor-ris水迷宫训练。结果Aβ2组、Aβ3组、I+NS组和S+NS组老年大鼠记忆质量下降(P<0.05),I+Aβ1组和S+Aβ1组老年大鼠空间记忆能力进一步下降(P<0.05);P+NS组与P+Aβ1组老年大鼠均表现出学习速度下降(P<0.05)。结论吸入麻醉药和脑室内Aβ1-40单体共同作用下进一步影响老年大鼠学习记忆功能,异丙酚却无此方面作用。
Objective To investigate the performance of Morris water maze after isoflurane/sevoflurane/ propofol anesthesia in encephalocoele injected rats. Methods One hundred 20-month old rats were randomly divided into 10 groups. 10 μL normal saline/Aβ1-40 monomer/solubility Aβ1-40 oligomer/insolubility Aβ1-40 fiber was injected into encephalocoele in rats （group control/Aβ1/Aβ2/Aβ3）. Rats in group I＋ NS/I＋ Aβ1 inhaled 1.3 MAC isoflurane for 4 h after the injection of NS or Aβ1-40 monomer. Rats in group S＋NS/S＋Aβ1 inhaled 1.3 MAC sevoflurane for 4 h after the injection of NS or Aβ 40 monomer. Rats in group P＋NS/P＋Aβ1 were anesthetized by propofol for 4 h after the injection of NS or Aβ1-40 monomer. All the rats performed Morris water maze （MWM） test to assess the cognitive function after 2 weeks. Results According to the performances of MWM, we found the cognitive function were decreased in rats of group Aβ2, Aβ3, I＋NS and S＋NS （P〈0.05）. The cognitive function was worse in rats of group I＋Aβ1 and group S＋AβI than that in group I＋NS and group S＋NS （P〈0.05）. The cognitive functions were remarkably decreased in rats of group P＋NS and P＋Aβ1 and there was no difference between them. Conclusion The spatial memory which was involved in learning and memory process was impaired and was inhibited by isoflurane/sevoflurane/propofol, solubility Aβ1-40 monomer and fibril formation. Under the joint-effect of anesthetics and Aβ1-40 monomer, the spatial memory of old rats was further impaired.
基金
国家自然科学基金(3057192)
关键词
麻醉药
全身
淀粉样Β蛋白
阿尔茨海默病
Anesthetics geheral
Amyloid beta-protein
Alzheimer＇s disease
