摘要
用Ｂ９５８细胞株产生的ＥＢ病毒，转化棉顶狨猴外周血淋巴细胞，获得ＫＭＴ３细胞株。该细胞株能产生高滴度的ＥＢ病毒。含ＥＢ病毒衣壳抗原的自然阳性细胞率为３～５％，激活后达５０～６０％，其培养上清液可直接转化人淋巴细胞得到传代细胞系。
A lymphoblastoid cell line (KMT 3) was established by EBV induced B cell transformation in the cotton top tamarin. The viruses were prepared from the culture fluid of B 95 8 cells by passing through a 0.45μm filter. KMT 3 can produce high titer infections EB viruses. Its VCA positive cell rate was 3～5% in natural and 50～60% after activation. Human B lymphocytes could be immortalized directly from KMT 3 supernatant. The number of chromosomes was 46.
出处
《中国病毒学》
CSCD
1998年第1期29-33,共5页
Virologica Sinica
关键词
淋巴细胞
EB病毒
细胞株
棉顶绒猴
Lymphoblastoid, Epstein Barr Virus, Transformation, Cell line, Cotton top tamarin
