摘要
在大鼠动情前期促黄体生成激素峰形成前后测定了血清雌、孕激素水平变化、下丘脑正中隆起促黄体生成激素释放激素含量及弓状核区雌、孕激素受体密度改变和弓状核前阿黑皮素ｍＲＮＡ水平变化。结果表明：动情前期１４小时时血清雌激素水平开始升高（Ｐ＜００５），于１６小时时达高峰。此时弓状核雌激素受体密度降低（Ｐ＜００５），孕激素受体密度增加（Ｐ＜００５），前阿黑皮素ｍＲＮＡ水平减少（Ｐ＜００５）。前阿黑皮素ｍＲＮＡ水平与血清雌激素及正中隆起的促性腺激素释放激素水平呈负相关（Ｐ值分别小于００１及００５），与弓状核雌激素受体密度变化呈正相关（Ｐ＜００１）。提示弓状核的前阿黑皮素ｍＲＮＡ水平在此过程中可能受雌激素及其受体调控，下丘脑β内啡肽合成减少可能参与了排卵前促性腺激素释放激素／促黄体生成激素峰的形成。
To examine change in gene expression of POMC and its relationship with changes in levels of serum estradiol, binding sites of estradiol, progesterone and GnRH densities in the hypothalamus during the preovulatory LH surge, time course studies were performed in pro estrous rats to determine the levels of serum estrogen, progesterone, GnRH densities in the median eminence(ME), binding sites of estrogen receptor(ER) and progesterone receptor(PR) and levels of Arc POMC mRNA in hypothalamic periarcuate area(Arc). The results showed that levels of serum estrogen increased at 1 400 h in the proestrous( P <0 05) and peaked at 1 600 h when levels of estrogen receptor binding sites and POMC mRNA in the Arc decreased( P <0 05) while Arc progesterone binding sites increased( P <0 05). There was a negative correlation between levels of Arc POMC mRNA and levels of serum estrogen or ME GnRH( P <0 01 and 0 05, respectively) and a positive correlation between levels of Arc POMC mRNA and estrogen binding sites( P <0 01). These results suggest that changes in gene expression of hypothalamic POMC during preovulatory LH surge may be regulated by estrogen and its receptors. The decreased synthesis of hypothalamic β END may be involved in the process of preovulatory GnRH/LH surge.
出处
《动物学报》
SCIE
CAS
CSCD
1998年第1期67-73,共7页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金
关键词
排卵前
前阿黑皮素
雌激素
受体
下丘脑
LH
GNRH
Preovulatory LH surge
Propiomelanocortin
Estrogen receptor
GnRH
Hypothalamus
Rat
