摘要
目的研究喉癌细胞的血管内皮生长因子(VEGF)表达及新型抗肿瘤药Avastin对喉癌细胞增殖的抑制作用。方法建立喉癌细胞的培养,对喉癌细胞VEGF进行免疫荧光染色,研究喉癌细胞的VEGF表达特性,利用Avastin对喉癌细胞的作用,采用四甲基偶氮唑蓝(MTT)法检测Avastin对喉癌细胞的抑制作用,Hoechst 33342染色法探讨其作用机制。结果喉癌细胞质中VEGF表达明显,Avastin对喉癌细胞的增殖抑制较对照组有统计学差异(P<0.05)。结论VEGF为喉癌细胞质内表达蛋白,Avastin对喉癌细胞有直接杀伤作用,新型抗肿瘤药物Avastin为喉癌的治疗提供了新的方法。
Objective To study the expression of vascular endothelial growth factor and evaluate the effect of Avastin to the inhibition of the cell on laryngeal squamous cell carcinoma. Methods Establishing the culture on laryngeal squamous carcinoma cell and laryngeal squamous carcinoma cell was observed with immunofluorescent histochemistry, the expression character of the laryngeal squamous carcinoma cell was studied. The effect of Avastin was detected by methyl thiazolyl terazolium（MTT） assay. Hoechst 33342 staining were adopted to explore the killing mechanism of Avastin. Results VEGF obvious expressed in the laryngeal squamous carcinoma cytoplasm. Compared with control group, Avastin inhibitive effects of the growth of laryngeal squamous cell carcinoma had the statistical difference （ P 〈 0.05 ）. Conclusions The protein of VEGF mainly expressed in laryngeal squamous carcinoma cytoplasm. Avastin had direct effects on laryngeal squamous cell in vitro. The method of theraping laryngeal squamous carcinoma was provided by Avastin of the the new-type antitumor drug.
出处
《山东医药》
CAS
北大核心
2009年第9期25-27,共3页
Shandong Medical Journal
基金
安徽省自然科学基金资助项目(070413115)。
关键词
喉肿瘤
鳞状细胞
AVASTIN
免疫荧光
血管内皮生长因子
laryngeal Neoplasms, squamous cell
Avastin
immunofluorescent
vascular endothelial growth factor
