摘要
目的观察七氟烷预处理对心肌缺血／再灌注大鼠心肌核因子-κB（NF-κB）p50表达的影响，进而探讨NF-κB在七氟烷预处理保护机制中的作用。方法SD雄性大鼠70只，随机分为7组。空白对照组；单纯缺血组；七氟烷组于吸入2．4％七氟烷30min，继之15min药物排出后进行心肌缺血／再灌注；七氟烷对照组吸入七氟烷30min后停止吸入165min；小白菊内酯（parthenolide，PTN）组于缺血前15min腹腔内注射NF-κB特异性抑制剂PTN500μg／kg；PTN＋七氟烷组于七氟烷预处理前15min腹腔内注射PTN500μg／kg；七氟烷＋PTN组于预处理后即刻腹腔内注射门N500μg／kg。建立大鼠心肌缺血，再灌注损伤的在体模型，阻断左冠状动脉前降支30min，再灌注120min，分别于大鼠心肌缺血／再灌注前、后或相应时间点取心肌标本。采用western blot法测定NF-κBp50的蛋白表达。结果缺血前七氟烷组较空白对照组NF-κBp50蛋白表达明显上调（P〈0．05）；缺血后与空白对照组比较，单纯缺血组、七氟烷组NF-κBp50蛋白表达显著增多（P〈0．05）；与单纯缺血组比较，七氟烷组NF-κBp50蛋白表达显著减少（P〈0．05）；缺血后七氟烷组较缺血前七氟烷组NF-κBp50蛋白表达显著减少（P〈0．05）.结论七氟烷预处理期间NF-κBp50大量激活，抑制了心肌缺血／再灌注后期NF-κBp50蛋白的表达，该作用被PTN所阻断，NF-κBp50可能参与了七氟烷预处理的心肌保护机制。
Objective To study the effects of sevoflurane preconditioning on the expression of nuclear factor-κB（NF-κB） pSO in rat myocardial ischemia/reperfusion （I/R）model. Methods Seventy male SD rats were randomly divided into seven groups： control group; simple-ischemic group： receiving I/R merely; sevoflurane group： inhaling 2.4 % sevoflurane for 30 min and then washing-out for 15 min followed by I/R 30min; sevoflurane control group： inhaling sevoflurane for 30 min merely; PTN group： receiving PIN 500 μg/kg （NF-KB inhibitor） 15 min before I/R; PTN＋sevoflurane group and sevoflurane＋PTN group： receiving PTN 500 μg/kg 15 min before and after sevoflurane preconditioning, respectively. Myocardium sample for all groups were collected before and after I/R or the corresponding time points. NF-κB p50 was detected using western blotting method. Results The expression of NF-κB p50 was significantly up-regulated in sevoflurane group before I/R （P〈0.05）. After I/R the expression of NF-κB pS0 was significantly higher in simple-ischemic group than that in control group （P〈0.05）, and was significantly lower in sevoflurane group than in simple-ischemic group （P〈0.05）. Conclusion NF-κB p50 may participate in the I/R injury and plays an important role in the protective mechanism of sevoflurane preconditioning.
出处
《国际麻醉学与复苏杂志》
CAS
2009年第2期112-115,共4页
International Journal of Anesthesiology and Resuscitation
基金
2008年江苏省自然科学基金资助项目（BK20080387）
关键词
七氟烷
预处理
心肌再灌注损伤
核因子-κB
P50
Sevoflurane
Preconditioning
Myocardial reperfusion injury
Nuclear factor-κB p50
