摘要
目的:就中国海域分离的创伤弧菌对小鼠的毒力、伤口感染力和对抗生素的敏感性进行实验研究.方法:昆明小鼠腹腔注射创伤弧菌悬液,观察一般状态、白细胞计数和血培养.第5日脱颈处死后解剖取脏器,用甲醛固定,石蜡包埋、制片,HE染色,光镜观察.在毒力实验中,昆明小鼠致伤后浸泡在含创伤弧菌的人工海水中45～60min,打捞后分笼于层流柜中饲养.于致伤浸泡后不同时间行白细胞计数及血培养,并观察一般状态和伤口炎性反应、定时取伤肢分泌物培养.将伤肢用甲醛固定,石蜡包埋、制片,HE染色,用光镜检查.用K-B法作创伤弧菌对各种抗生素药物敏感性实验.结果:小鼠注射创伤弧菌悬液后白细胞升高,死亡率为80%,小鼠的血、脾、肺、肝、肾、肠组织及腹腔培养物细菌培养均为阳性,病变部位以肺、肝及肾脏为重,病变性质主要为血液循环障碍,并由此引起不同程度的充血、出血及血停滞现象.海水浸泡伤创伤弧菌感染小鼠死亡率为20%;20%小鼠12h血培养阳性;6,12h及1～3d伤肢培养均为阳性,死亡小鼠及1d后活杀小鼠所有脏器培养均为阳性.感染小鼠的脏器可见到不同程度的炎症反应,以肺和肝脏较为明显,并有脓肿灶形成.结论:中国海域分离的创伤弧菌具有高度致病力,创伤弧菌除对氨苄西林、哌拉西林、氨曲南、磺胺类抗生素的敏感性较低外,对其它抗生素均有很好的抗菌活性.
AIM： To conduct experimental studies on the toxic infection ability and antibiotic sensitivity of Vibrio Vulnificus isolated from the maritime space of China. METHODS： The general status, leukocyte count and blood culture of Kunming mice were examined after the mice were intraperitoneally injected with Vibrio Vulnificus suspension. Five days＇ after the injection, the mice were sacrificed and he organs of the sacrificed mice were fixed with formalin fixation, imbedded in paraffin, stained with HE and observed by light microscope. In toxicity experiment, vulnerant Kumning mice were soaked in artificial sea water for 45 minutes to 60 minutes. The mice were taken out from the water after different time points. The general status, leukocyte count and blood culture were examined, and the antibiotic susceptibility of Vibrio Vulnificus was tested by traditional K-B methods. RESULTS ： After injection of Vibrio Vulnificus, the leukocytes of the mice increased and the mortality rate of mice was 80%. The tests of blood, spleens, lungs, livers, kidneys, intestines and carum abdominis cultures were all Vibrio Vulnificus positive. The diseased regions of lungs, livers and kidneys were severer than those in other organs, and hyperemia, hemorrhage and stasis were observed because of dysaemia. The mortality rate of Vulnerant and soaked Kunming mice was 20% and the positive rate of blood culture was 20%. The tests of cultures from secretions of injured extremity after 6 h, 12 h and dayl to day 3 were all positive and the organ cultures also showed positive results. Inflammatory reaction was observed in organs of infected mice and it was severer in lungs and livers, where abscess was seen. CONCLUSION： Vibrio Vulnificus isolated from maritime space of China is highly virulent. Despite its lower sensibility to Ampicillin, Piperacillin, Aztrconam and sulfanilamide groups, this Vibrio Vulnificns appears to be well sensitive to other antibiotics.
出处
《第四军医大学学报》
北大核心
2009年第6期493-496,共4页
Journal of the Fourth Military Medical University
基金
军队医药卫生科研基金重点项目(总后20051102)
军队"十一五"专项基金(06Z007)
关键词
中国海域
弧菌
创伤
毒力
抗菌药
maritime space of china
Vibrato vulnificns
patho- genicity
antibiotic
