摘要
细胞培养稳定同位素标记技术(SILAC)是在细胞培养过程中,利用稳定同位素标记的氨基酸结合质谱技术,对蛋白表达进行定量分析的一种新技术。它不仅可以对蛋白质进行定性分析,还可通过质谱图上一对轻-重稳定同位素峰的比例来反映对应蛋白在不同状态下的表达水平,实现对蛋白质的精确定量。SILAC结合质谱技术在定量蛋白质组学中发挥了巨大的作用,其应用范围从细胞系扩展到亚细胞器、组织与动物整体水平,具体的应用策略也在不断完善发展。我们总结评述了SILAC技术在差异表达蛋白质组、蛋白质翻译后修饰、药物蛋白质组和蛋白质相互作用等方面的应用与进展。
The SILAC（stable isotope labeling with amino acids in cell culture） technique in combination with mass spectrometry（MS） can take quantitative measurement of the expressing protein in different states according to the ratio of light to heavy isotopic peak derived from the labeling protein. It has played an important role in the field of quantitative proteomies. The application of SILAC has become more and more extensive, and its specific strategies keep renewing. This paper has made a summary of the application and development of SILAC on differential expression proteome, protein posttranslational modification, pharmaceutical proteome and protein interactions, and so on.
出处
《生物技术通讯》
CAS
2009年第2期238-242,共5页
Letters in Biotechnology
基金
国家自然科学基金(20505006)
关键词
细胞培养稳定同位素标记
定量蛋白质组学
质谱
stable isotope labeling with amino acids in cell culture（SILAC）
quantitative proteomies
mass spectrometry
