摘要
目的:分离主导管结扎后损伤的下颌下腺中涎腺干/祖细胞,并对其特征进行研究。方法:结扎SD大鼠下颌下腺主导管,获得损伤模型;机械及酶消化法体外分离、培养腺体细胞,获得类上皮细胞集落;挑取集落后梯度稀释法纯化获得类上皮单克隆细胞(涎腺干/祖细胞,salivary gland progenitor cells,SGP);利用α6β1整合素、层粘蛋白、β1整合素、角蛋白19分别进行免疫细胞化学、免疫荧光染色鉴定。结果:SGP细胞α6β1整合素、层粘蛋白、β1整合素、角蛋白19抗体阳性。结论:主导管结扎后损伤的下颌下腺中能分离出具有组织干细胞特征的细胞。
Objective：To isolate salivary gland progenitor cells from damaged submandibular gland and to investigate the characteristics of the cells. Methods： Tissue trauma was performed by ligation of main duct in SpragueDawlye （SD） rats. Cells isolated by mechanical method and enzyme digestion. With limited dilution, cell lines purified from epithelium-like colonies were designated as salivary gland progenitor cells-SGP and their characterizations were analyzed by antibodies of α6β1 integrin, laminin, integrinβ1 , CK 19 with immunochemistry. Results ： The SGP expressed α6β1 integrin, cytoplasmic laminin, integrinβ1 and cytokeratin19. Conclusion： The cells isolated from damaged submandibular gland shows characteristics of tissue stem cells.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2009年第2期174-177,共4页
Journal of Practical Stomatology
基金
贵州省科学技术厅科技攻关基金项目〔(2003)53号〕
贵州省优秀科技教育人才省长资金〔黔省专合字(2008)113号〕
贵州省教育厅基金项目〔(2006)354号〕
关键词
损伤下颌下腺模型
涎腺干/祖细胞
分离
培养
Damaged Submandibular Gland
Salivary Gland Progenitor Cells
Isolation
Culture
