摘要
目的探讨快速老化小鼠听觉功能、以及耳蜗组织中甲基化结合蛋白-2(methyl-CpG-bindingprotein2,MeCP2)的增龄性变化。方法本研究选3、5、7、9月龄的快速老化小鼠亚系1(Senescence acceler-ated mouse/prone1,SAMP 1)作为实验组,而同龄抗快速老化亚系1(Senescence accelerated mouse/resistance 1,SAMR 1)作为SAMP 1的正常对照组。应用听觉诱发反应仪检测其听觉脑干反应阈值;应用RT-PCR和Western印迹杂交对耳蜗组织中MeCP2mRNA和蛋白的表达水平进行半定量分析。结果第7、9月龄SAMP1脑干反应阈值较同龄SAMR1明显增高(P<0.05);MeCP2 mRNA和蛋白在不同月龄快速老化小鼠耳蜗组织中均有表达,第7、9月龄SAMP1小鼠耳蜗组织中的MeCP2 mRNA和蛋白较同龄SAMR1小鼠明显降低(P<0.05)。结论MeCP2 mRNA和蛋白的表达水平随着快速老化小鼠听觉功能增龄性减退而降低,说明MeCP2可能与维持耳蜗的功能状态有关。
[Objective] The purpose of this study was to analyze age-related hearing loss in the senescence accelerated mouse and the expression of MeCP2 gene at mRNA and protein in the cochlea of the senescence accelerated mouse throughout development. [Methods] We chose the 3, 5, 7, 9th month as analyzing time points. The auditory thresholds and the expression of MeCP2 gene at mRNA and protein in the cochlea were studied in the senescence accelerated mouse/prone 1 （SAMP 1） at 3, 5, 7, 9 months. Normal aging Control the senescence accelerated mouse/resistance 1 （SAMR 1） was used. The expression of MeCP2 gene at mRNA level was analyzed by RT-PCR. The expression of MeCP2 protein was analyzed by Western blot. [Results] Auditory function evaluation： Compared with the SAMR 1, the SAMP 1 at 7, 9 months developed a progressive hearing loss at 8 kHz,which showed an agerelated significant increase （P 〈0.05）. The expression of MeCP2 mRNA and MeCP2 protein in the cochlea： there were MeCP2 mRNA and MeCP2 protein expressed in the cochlea of the senescence accelerated mouse throughout the development. Compared with the SAMR 1, the SAMP 1 at 7, 9 months developed a significant descend （P 〈 0.05）. [Conclusion] The expression level of MeCP2 mRNA and MeCP2 protein decreases when the senescence ac- celerated mouse develop a progressive hearing loss. This indicates that MeCP2 protein probably has relationship with maintaining functional status of the cochlea.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第5期687-690,共4页
China Journal of Modern Medicine
基金
教育部春晖计划科研项目(No:Z2005-2-45002)
广西卫生厅科研项目(No:200746和Z2007193)
关键词
快速老化小鼠
听觉脑干反应阈值
耳蜗
甲基化结合蛋白-2
senescence accelerated mouse
threshold of auditory brainstem response
cochlea
methyl-CpG- binding protein 2
