摘要
采用药浴方式,研究了一种新型消毒剂——聚六亚甲基双胍对凡纳滨对虾受精卵、无节幼体、幼虾、成虾的毒性及饵料藻类的影响。结果表明,聚六亚甲基双胍对凡纳滨对虾幼虾24、48h的LC50为64.1mg·L-1和31.13mg·L-1,安全浓度Sc为2.20mg·L-1;聚六亚甲基双胍对凡纳滨对虾成虾24、48h的LC50为66.17mg·L-1和32.16mg·L-1,安全浓度Sc为2.28mg·L-1,为了保证凡纳滨对虾的安全性,聚六亚甲基双胍的用量应控制在0.5mg·L-1之内;聚六亚甲基双胍在125mg·L-1浓度内对受精卵的孵化率无明显影响,0.5mg·L-1浓度以内对无节幼体的变态率无明显影响。聚六亚甲基双胍对球等鞭金藻和亚心形扁藻表现出了明显的剂量-效应关系,其对球等鞭金藻和亚心形扁藻的生长不产生抑制的最高浓度为5mg·L-1;聚六亚甲基双胍对球等鞭金藻48、96、144h的EC50为27.01、34.95mg·L-1和33.14mg·L-1;亚心形扁藻为34.65、28.73mg·L-1和20.57mg·L-1,聚六亚甲基双胍对两种藻类的安全浓度分别3.5mg·L-1和2.9mg·L-1,表明聚六亚甲基双胍对亚心形扁藻的影响更明显。
The security of polyhexamethylene biguanide（PHMB ）on different growth stages of Litopenaeus vannamei and feeding algae were investigated. The results showed：cumulative mortality of juvenile and adult Litopenaeus vannamei increased with increasing concentration of PHMB. 24 h and 48 h median lethal concentrations （LC50） of PHMB on juvenile shrimp of Litopenaeus vannamei were 64.1 mg·L^-1 and 31.13 mg·L^-1, and security concentration（ Sc ）was 2.20 mg·L^-1. 24 h and 48 h median lethal concentrations（LC50）of PHMB on adult shrimp of Litopenaeus vannamei were 66.17 mg·L^-1 and 32.16 mg·L^-1, and security concentration（ Sc ）was 2.28 mg·L^-1. In order to guarantee security on cuhuring Litopenaeus vannamei, the using amount of PHMB should below 0.5 mg·L^-1. Hatching and metamorphosis test showed ： hatching rate of control group was 87%, and hatching rate of test group was the range of 79%-92%. There was no significant influence on hatching rate of zygote when the concentration of PHMB below 125 mg·L^-1, and there was no significant influence on metamorphosis rate of nauplius when the concentration of PHMB below 0.5mg·L^-1. With the increase of PHMB concentration, the growth inhibition rate of Isochrysis galbana and Platymonas subcordiformis was increasingly apparent. There was an excellent relationship between dose and effect of PHMB on lsochrysis galbana and Platymonas subcordiformis, and the sensitivity of Platymonas subcordiformis to PHMB is higher than Isochrysis galbana. The highest concentration of no significant influence of PHMB on lsochrysis galbana and Platymonas subcordiformis was 5 mg·L^-1. 48 h, 96 h and 144 h EC50 of PHMB on Isochrysis galbana were 27.01, 34.95 mg·L^-1 and 33.14mg·L^-1, and the corresponding concentrations of Platymonas subcordiformis were 34.65,28.73mg·L^-1 and 20.57mg·L^-1. According to the classification of aquatic organisms toxicity, the toxicity of PHMB to Isochrysis galbana and Platymonas subcordiformis belonged to moderate toxicity.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2009年第3期597-601,共5页
Journal of Agro-Environment Science
基金
国家科技支撑计划"工程化养殖高效生产体系构建技术研究与开发"(2006BAD09A03)
公益性行业科研专项"对虾养殖管理信息系统研究与建立"(nyhyzx07-042)
关键词
凡纳滨对虾
聚六亚甲基双胍
饵料藻类
安全性
Litopenaeus vannamei
polyhexamethylene biguanide（ PHMB ）
feeding algae
security
