摘要
目的:探讨胃癌病人血清中血管内皮生长因子(VEGF)与胃癌组织中微血管密度(MVD)关系,及其与胃癌临床病理特征的关系。方法:采用酶联免疫法检测42例胃癌病人和正常对照组20例血清中VEGF浓度。应用鼠抗人CD34单克隆抗体进行免疫组织化学染色检测胃癌组织中MVD。结果:胃癌病人术前VEGF浓度高于正常对照组(P<0.01),胃癌病人癌组织中MVD高于自身正常胃组织中MVD,且具有显著性差异(P<0.01);胃癌病人术前VEGF浓度与胃癌组织中MVD呈正相关(r=0.875),胃癌病人血清中VEGF浓度及癌组织中MVD与胃癌浸润深度(P<0.01)、淋巴结转移(P<0.05)以及组织学分型有显著性差异(P<0.05),与其年龄、性别无关(P>0.05)。结论:胃癌的血管生成与血清中VEGF浓度密切相关,胃癌病人血清中VEGF浓度有可能作为胃癌疗效评价、复发、转移及预后的重要指标。
Objective：To study the relationship between serum vascular endothelial growth factor（VEGF）,microvessel density（MVD） and their clinical pathologic feature in patients with gastric cancer.Methods：Enzyme-linked immunosorbent assay（ELISA） and immunohistochemical staining were used to detect serum VEGF concentration and MVD of tumor tissue in 42 cases with gastric cancer.Use mouse ante-human mocolonal antibody to detecte the MVD in castric carcinoma with immunohis to chemistry.Results：Serum VEGF was found to be significantly higher in patients with gastric cancer than that in control group.More significant difference was observed in MVD of tumor tissue than that of normal tissue in patients with gastric cancer（P〈0.01）.There were positive correlation between VEGF and MVD in patients with gastric cancer（r=0.875）.VEGF in serum and MVD in tumor tissue of gastric cancer were correlated with lymph node metastasis（P〈0.05）,depth of invasion（P〈0.01） and histological stage（P〈0.05）,but there was no relations to age and sex（P〉0.05）.Conclusion：VEGF is significantly related to angiogenesis of gastric cancer,Serum VEGF of gastric cancer may be an important index for evaluation of recurrence,metastasis and prognosis for patients.
出处
《内蒙古医学院学报》
2009年第1期13-18,共6页
Acta Academiae Medicinae Neimongol
