摘要
目的评价一种新的基因样本获取办法用于大样本遗传学研究。方法通过邮寄的方法获得病人的漱口液(mouthwash)共492份,用氯-酚法提取其中脱落的口腔上皮细胞中的DNA,测定提取数量;通过聚合酶链反应(PCR)用提取的DNA扩增大分子红视蛋白DNA片段,并将纯化的DNA产物进行转化生长因子β诱导因子的部分片段基因测序,以评定DNA提取质量。结果每份样本的DNA提取量中位数为52.63μg(10%及90%界值分别为16和143.25μg),其中10～20ng的DNA即可获得好的PCR扩增产物,并能得到清晰的基因测序结果。结论用邮寄的方法,采用生理盐水做漱口液,采集口腔黏膜上皮细胞,从中提取的人类DNA的数量和质量均理想,在实际应用中结果可靠。
Objective To evaluate a new approach to genetic collection for large samples genetic study. Methods 492 mouthwashes were acquired by mail. DNA in exfoliated oral mucosal epithelial cells was extracted with Chlore-phenol method and its amount was measured. DNA segments of red opsin were amplified with extracted DNA by PCR, and partial segments of transforming growth factor β induced factor in purified DNA were tested for Sequence to estimate the quality of extracted DNA. Results The median of extracted DNA in each sample was 52. 63 （g（ 10% and 90% critical value were 16 and 143.25 （g respectively）. Good PCR production was acquired with 10 -20 ng DNA, and clear gene sequencing consequence was obtained. Conclusions The quantity and quality of DNA extracted from oral mucosal epithelial ceils in saline mouthwashes by mail are ideal, which is reliable in practice.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第6期678-681,共4页
Chinese Journal of Gerontology
基金
教育部留学回国人员科研启动基金资助项目(2005)
山东省优秀中青年科学家科研奖励基金(2007BS03056)
