摘要
通过25个多态性较好的微卫星标记,检测我国26个地方鹅品种1 560个个体,共检测到198个等位基因,每个位点平均为7.92个。有效等位基因数为1.307 0～5.795 5,平均3.066 2个。就全群而言,平均观察杂合度、期望杂合度、多态信息含量分别为0.668 6、0.457 7和0.564 0。对各个群体而言,观察杂合度、期望杂合度和多态信息含量变异范围分别是0.535 1～0.754 7、0.383 1～0.604 9、0.324 0～0.538 0。群体间存在明显的遗传分化,约有28%的遗传分化来自于群体间的变异。右江鹅与永康灰鹅之间的Reynolds遗传距离最大（0.522）,基因流值最小（0.365）,武冈铜鹅与豁眼鹅之间的Reynolds遗传距离最小（0.133）,基因流值最大（1.763）。在Reynolds遗传距离的基础上做品种的NJ聚类图,26个地方鹅资源群体分为5个类群。研究结果为我国地方鹅种种质特性研究提供基础数据,为我国地方鹅品种资源的合理保护和利用提供科学依据。
The genetic diversity and differentiation of twenty six Chinese native goose populations were investigated using 25 microsatellite markers. The total number of animals examined was 1 560. A total of 198 alleles were observed, with mean number 7.92 alleles per locus. Effective alleles number varied from 1. 307 0 to 5. 795 5, with an average of 3. 066 2. The polymorphic information content （PIC） ranged from 0. 324 to 0. 538, the observed heterozygosity （Ho） ranged from 0. 535 1 to 0. 754 7, and the expected heterozygosity （He） ranged from 0. 383 1 to 0. 604 9. As a complete populations, the average Ho, H, and PIC were 0. 668 6, 0. 457 7 and 0. 564 0, respectively. Considerable genetic differentiation was observed and 20.7 % of the total genetic variation originated from the differences among breeds. The largest Reynolds genetic distance and lowest gene flow were between Youjiang and Yongkang grey geeses, meanwhile, the nearest Reynolds genetic distance and highest gene flow were between Wugangtong and Huoyan geeses. Consensus tree was constructed using the Neighbour-Joining method based on the Reynolds ge- netic distance, and five groups were found. The results may provide basic molecular data for the research on the germplasm characteristics of native goose breeds in China and scientific basis for the conservation and utilization of those breeds.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第3期327-332,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家科技支撑计划(2008BADB2B08)
"国家科技基础条件平台"资助(2005DKA21101)
江苏省科技服务平台(BM2008170)资助
关键词
鹅
微卫星标记
遗传变异
遗传分化
geese
microsatellite markers
genetic variation
genetic differentiation
