摘要
目的:建立同时测定地奥心血康胶囊中原薯蓣皂苷、甲基原薯蓣皂苷和薯蓣皂苷3种成分含量的HPLC-ELSD分析方法。方法:采用Shim-pack VP-ODS色谱柱(250mm×4.6mm,5μm),以乙腈(A)-水(B)(0～15min,B:75%→71%;15～25min,B:71%;25～45min,B:71%→65%;45～65min,B:65%→40%;66min,B:30%;66～75min,B:30%→0%)为流动相进行梯度洗脱,流速为0.8mL·min-1,柱温为35℃。采用蒸发光散射检测器,漂移管的温度为105℃,气体(空气)流速为2.7L·min-1。结果:原薯蓣皂苷、甲基原薯蓣皂苷和薯蓣皂苷的线性范围分别为1.021～10.21μg(r=0.9999),1.029～10.29μg(r=0.9995)和1.074～10.74μg(r=0.9998),平均回收率(n=9)依次为99.2%(RSD=2.0%),101.9%(RSD=1.5%)和100.7%(RSD=2.0%)。结论:本方法简便、准确、重现性好,可用于地奥心血康胶囊中原薯蓣皂苷、甲基原薯蓣皂苷和薯蓣皂苷的含量测定。
Objective：To develop a new quantitative analysis method for the simultaneous determination of protodioscin,methylprotodioscin,and dioscin in Di＇aoxinxuekang capsules by HPLC-ELSD.Methods：A Waters Allinace 2695 HPLC（Empower workstation）was used with Alltech 2000 ELSD as detector.The samples were separated with a Shim-pack VP-ODS column（250 mm×4.6 mm,5 μm.）using acetonitrile（A）and water（B）under gradient conditions（0-15 min,B：75%→71%;15-25 min,B：71%;25-45 min,B：71%→65%;45-65 min,B：65%→40%;66 min,B：30%;66-75 min,B：30%→0%）.The flow rate was 0.8 mL·min-1 and the column temperature was 35 ℃.The drift tube temperature of the evaporative light-scattering detector was 105 ℃ and clear air flow rate：2.7 L·min-1.Results：The linear ranges protodioscin,methylprotodioscin,and dioscin were 1.021-10.21 μg（r=0.9999）,1.029-10.29 μg（r=0.9995）and 1.074-10.74 μg（r=0.9998）,respectively.The average recoveries（n=9）of protodioscin,methylprotodioscin,and dioscin were 99.2%（RSD = 2.0%）,101.9%（RSD=1.5%）and 100.7%（RSD = 2.0%）,respectively.Conclusion：Protodioscin,methylprotodioscin,and dioscin in Di＇aoxinxuekang capsules can be simultaneously determined by this method established which is simple,accurate and reproducible.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第3期420-422,共3页
Chinese Journal of Pharmaceutical Analysis
