摘要
目的目的建立测定人血浆中丁螺环酮浓度的高效液相色谱法。方法以DiamonsilTMC18反相柱(150mm×4.6mm,5μm),为色谱柱,流动相为0.03mol.L-1;醋酸铵-甲醇(22∶78);流速:0.8mL·min-1;柱温40℃;检测波长:238nm。以乙酸乙酯与二氯甲烷(80∶20)为提取剂。结果丁螺环酮的高、中、低(400.0,80.0,10.0ng·mL-1)3种浓度平均回收率分别为99.28%,97.63%,102.41%,日内、日间差RSD均低于7%(n=5);分析方法的最低检测限为5.0ng·mL-1;线性范围为10.0～400.0ng·mL-1。曲线方程:Y=65.69X+1.87,r=0.9993(n=9)。结论该方法灵敏、准确、简单、快速,可用于临床血药浓度监测和药动学研究。
OBJECTIVE To establish a method for determining the concentration of buspirone in human plasma by HPLC. METHODS The drug from plasma analyzed in a reverse phase HPLC system（C18 column, 150mm × 4.6mm, 5μm） ; mobile phase consisted of 0.03mol·L^-1 ammonioum-methanol （22 ： 78） ; the flow rate was 0. 8mL· min^-1 ;The detection wavelength was at 238nm. Ethyl acetate and dichloromethane（80 ： 20）was used as extracting solvent. RESULTS The average recoveries of buspirone in high, mediun and low concentrations（400.0, 80.0, 10.0ng·L^-1 ）were 99.28 %, 97.63 % and 102.41%, respectively. The within-day and between-day precision of variation was less than 7 %. The minumum detectable concentration of buspirone,was 5.0ng·L^-1. The calibration curves of buspirone showed good linearity, over the range of 10.0 -400.0ng·L^-1. The regression equation was Y = 65.69X ＋ 1.87, r = 0. 9993 （n = 9）. CONCLUSION The method provides a sensitive accurate, easy and quick, which can be used for clinical drug monitoring and pharmacokinetic studies.
出处
《海峡药学》
2009年第1期93-95,共3页
Strait Pharmaceutical Journal
