摘要
基因(组)操作者常会遇到高GC序列难于扩增的问题。全球范围内也还没有很成熟的通用方法来解决这个问题。经过系统的摸索,发现选用有机试剂乙二醇和1,2-丙二醇能得到比较满意的特异的PCR产物。104段随机选取的GC含量在60%～80%之间的人类基因组序列(长度在700～800bp)基本上全部得到较好的扩增。
Investigators in gene （genome） manipulation frequently meet difficulty in amplification of GC-rich DNA sequences. This difficulty has not been well solved worldwide. Through systematic screening, 1,2- propanediol and ethylene glycol were found specifically efficient in enhancing the amplification of high GC content human genomic DNA. Randomly selected 104 GC-rich sequences （GC content from 60% to 80% and length from 700 to 800bp） from human genomic DNA to test the two organic solvents and all 104 amplicons were well amplified.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2009年第3期69-73,共5页
China Biotechnology
基金
国家自然科学基金(30570401)
教育部新世纪优秀人才计划(2006)
TUST基金(20050422
20060418)资助项目
