摘要
目的研究鸦胆子乳剂、5-Fu局部瘤体注射联合灌服开道散对人胃癌SGC-7901移植瘤的抑制作用及可能机制。方法建立人胃癌SGC-7901裸鼠移植瘤模型后传3代,接种于裸小鼠右前肢腋窝皮下,待肿瘤生长至50-250mm3后随机分6组:对照组A组(0.9%NaCl溶液),治疗组B(5-FU)组、C组(5-FU及鸦胆子乳)、D组(开道散联合5-FU)、E组(开道散联合5-FU、鸦胆子乳)、F组(开道散)。实验期间,每周测量两次瘤体体积,给药结束,将动物脱颈处死,剥离出肿块称重,比较各组肿瘤重量,计算肿瘤抑制百分率;用免疫组化方法检测荷瘤组织中p53、Ki67表达情况。结果除F组瘤重与对照组比较无统计学意义(P>0.05),余各治疗组瘤重均显著低于对照组,但治疗组间C组、D组与B组比较无统计学意义(P>0.05),而E组与B组相比有统计学意义(P<0.05);除F组外,其余各治疗组均有不同程度下调p53、Ki67表达,与对照组比较差异有统计学意义(P<0.05),但C组、D组与B组比较各免疫组化指标表达无明显差异(P>0.05),而E组较B组各免疫组化指标下调明显,有统计学意义(P<0.05)。结论瘤体内注射鸦胆子乳剂、5-Fu联合灌服开道散有一定的协同增效作用,其机制可能与抑制p53、Ki67表达,抑制肿瘤细胞的生长与增殖有关。
Objective To investigate the inhibitory effect of local Bruce a javanica and 5-fluorouracil（5-Fu） injection combined with Kaidao powder on the implanted human gastric tumor SGC-7901 in nude mice and to explore its mechanism. Methods Establish the model of human gastric tumor SGC-7901, after subculture for three generations, the tumor tissues were inoculated in subcutaneous part of right forelimb armpit in nude mice, when the tumor grew to the size of 50 -250mm^3 ,the mice were randomized into 6 groups： control group A （N. S）, treatment groups： group B（ 5-Fu ）, group C （5-Fu, Brucea javanica）, group D （Kaidan powder,5-Fu）, group E （5-Fu, Brucea javanica, Kaidao powder）, F（ Kaidao powder）. During the treatment, measure the volume of tumor twice a week; after experiment, all mice were sacri- lice, weigh and compare the tumor, calculate tumor growth inhibition rates of each group, and the expression of p53 and Ki67 examined by method. Results Except group F, the other groups＇ average tumor weight were lower than the control group, they were all more significant than the control group （ P 〈0.05 ）, but groups C and B, group D and B had no significance （ P 〉0.05 ）, group E contrast B had a significance（ P 〈0.05） ; except group F, the expression of p53 and Ki67 of the other groups were significantly lower than that of control group （ P 〈0.05） ,but eontrd group C and D with B,the difference were not significant （ P 〉0.05 ） ,though the difference between groups E and D was significant （ P 〈0.05 ）o Conclusion Local Bruce a javanica and 5-fluorouracil （5-Fu） injection combined with Kaidan powder has a synergistic action, and the growth inhibition may be via the inhibition of the expression of p53 and Ki67.
出处
《上海中医药杂志》
2009年第3期69-72,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
江苏省无锡市科学技术局社会发展项目(CLZ00626)
