摘要
目的:观察脑源性神经营养因子(BDNF)对小鼠未成熟卵的体外成熟及发育能力的影响。方法:以α-MEM为基础培养液,添加不同浓度(0、1、5、10 ng/ml)的BDNF以及FSH、FBS培养小鼠未成熟卵,并进行体外受精,观察卵母细胞成熟率、受精率和胚胎发育至囊胚的能力,了解不同培养条件下BDNF对卵母细胞发育能力的影响。结果:当体外成熟培养液中含有FSH和10%的FBS时,与体外成熟对照组比较,BDNF虽然不影响卵母细胞的成熟率和受精率,但BDNF 5 ng/ml组的囊胚形成率(75.00%)显著高于体外成熟对照组(56.63%),而接近体内成熟组(76.92%);当培养液中仅含FBS时,各组间卵母细胞成熟率和受精率没有差异,但与对照组比较,BDNF显著提高囊胚形成率;当培养液中不含FBS、FSH时,虽然无囊胚形成,但BDNF显著提高了卵母细胞的受精率。结论:BDNF能促进小鼠未成熟卵胞质的发育,提高卵母细胞的发育能力。
Objective： To observe the effect of brain - derived neurotrophic factor （BDNF） on maturation in vitro and developmental competence of murine immature oocytes. Methods： Murine immature oocytes were cultured in minimum essential media - αsupplemented with different concentration of BDNF （0, 1ng/ml, 5ng/ml, 10ng/ml）, FBS and FSH. After oocytes arrive at M Ⅱ stage, they were fertilized in vitro. It was observed for maturation rate, fertilization rate and blastocyst developmental rate of murine ooeytes. Results： Blastoeyst developmental rate in the group of 5ng/ml BDNF （75.00%） was higher than those in the control of oocytes matured in vitro （56. 63% ）, and it was similar with the group of ooeytes matured in vivo （76. 92% ） in the presence of 10% FBS and FSH in the media though maturation rate and fertilization rate of all groups were similar. Supplementation with BDNF （Sng/ml） during maturation in vitro significantly （ P 〈 0. 05 ） increased blastocyst development rate when the media was only with FBS. No blastoeyst was developed when the media was not including FBS and FSH, but BDNF significantly increased fertilization rate of oocytes. Conclusion： BDNF can promote cytoplasmic maturation of the ooeyte, which is essential for successful ooeyte development into preimplantation embryos.
出处
《中国妇幼保健》
CAS
北大核心
2009年第8期1106-1108,共3页
Maternal and Child Health Care of China
关键词
脑源性神经营养因子
卵母细胞
体外成熟
Brain- derived neurotrophic factor
Oocytes
Maturation in vitro
