摘要
目的检测乙型肝炎病毒携带者的精子是否携带乙肝病毒。方法12例乙型肝炎病毒携带者的精子经辅助生育技术洗涤后制成精子涂片,应用特异性乙肝病毒DNA(HBV DNA)的探针以原位杂交法检测这些患者的精子涂片。结果12例患者中有3例精子中检测到HBV DNA,分布于精子的核心部。结论经辅助生育技术洗涤后的患者的精子仍存在向子代传播乙肝的可能性。
Objective： To detected HBV DNA of spermatozoa samples from patients with HBV. Methods： Spermatozoa smears were made by washing samples from the twelve patients with chronic hepatitis B asking assisted reproductive techniques of our center, and were detected in situ hybridization using Dig - labeled with HBV DNA probe. Results ： Three of the spermatozoa samples got from 12 cases of patients with chronic hepatitis B were HBV DNA positive, which were found to be located at the cores of spermatozoa heads. Conclusion： It was possible that HBV infection could be vertically transmitted via infected spermatozoa after washing by assisted reproductive techniques.
出处
《中国优生与遗传杂志》
2009年第3期117-118,128,共3页
Chinese Journal of Birth Health & Heredity
关键词
精子
乙型肝炎病毒
原位杂交
Spermatozoa
Hepatitis
B virus In situ hybridization
