摘要
YN80 was isolated from Amorphophallus rivieri Durieu showing mosaic and crinkle symptoms in Songming,Yunnan province.Flexuous filamentous particles were found in diseased leave sap and pinwheel inclusion bodies were found in the leave tissue.YN80 had positive reaction to universal antibody of Potyvirus by DAS-ELISA.3’-terminal sequence of YN80 was cloned and sequenced.cp gene of YN80 consisted of 987 nt,encoded 328 aa(36.1 kDa).Sequence analysis showed that YN80 shared the highest identity(97.0%)with CP amino acid sequence of Dasheen mosaic virus(DsMV).These data indicated that YN80 was an isolate of DsMV.This is the first molecular identification of A.rivieri Durieu isolate of DsMV in China.
YN80 was isolated from Amorphophallus rivieri Durieu showing mosaic and crinkle symptoms in Songming, Yunnan province. Flexuous filamentous particles were found in diseased leave sap and pinwheel inclusion bodies were found in the leave tissue. YN80 had positive reaction to universal antibody of Potyvirus by DAS-ELISA. 3＇-terminal sequence of YNS0 was cloned and sequenced, cp gene of YNS0 consisted of 987 nt, encoded 328 aa （36.1 kDa）. Sequence analysis showed that YN80 shared the highest identity（97.0% ） with CP amino acid sequence of Dasheen mosaic virus （DsMV）. These data indicated that YN80 was an isolate of DsMV. This is the first molecular identification of A. rivieri Durieu isolate of DsMV in China.
出处
《植物病理学报》
CAS
CSCD
北大核心
2009年第1期88-90,共3页
Acta Phytopathologica Sinica
基金
科技部农业科技成果转化资金项目(02EFN215300560)
